SFRP1 蛋白, Mouse (CHO, His)

SFRP1 Protein is a secreted glycoprotein member of the SFRP family.SFRP1 Protein can act as an inhibitor of Wnt signaling and is capable of resisting vascular cell proliferation.SFRP1 Protein, Mouse (CHO, His) is the recombinant mouse-derived SFRP1 protein, expressed by CHO , with C-10*His labeled tag.

SFRP1 蛋白是 Wnt 信号的抑制剂，能抑制 WNT1/ WNT4 介导的 TCF 依赖性转录。SFRP1 Protein 可以降低细胞内 β-连环蛋白水平。SFRP1 Protein 在体外和体内对血管细胞有抗增殖作用，并能在体内诱导血管生成反应。在血管细胞周期中，延缓 G1 期和进入 S 期。在肾脏发育中，抑制后肾小管的形成和芽的生长。SFRP1 Protein 在维持造血干细胞 (HSC) 功能中起关键作用。SFRP1 蛋白是细胞间通讯的多功能调节剂，可以做为治疗神经退行性疾病中慢性炎症的潜在靶点^[1][2][3][4]。

Measured by its ability to inhibit Topflash reporter activity in HEK293T human embryonic kidney cells. The ED₅₀ for this effect is typically 0.4-2 μg/mL in the presence of 300 ng/mL of Recombinant Mouse Wnt-3a.

Mouse

CHO

C-10*His

AAC53145.1 (S32-K314)

20377  [NCBI]

SEYDYVSFQSDIGSYQSGRFYTKPPQCVDIPVDLRLCHNVGYKKMVLPNLLEHETMAEVKQQASSWVPLLNKNCHMGTQVFLCSLFAPVCLDRPIYPCRWLCEAVRDSCEPVMQFFGFYWPEMLKCDKFPEGDVCIAMTPPNTTEASKPQGTTVCPPCDNELKSEAIIEHLCASEFALRMKIKEVKKENGDKKIVPKKKKPLKLGPIKKKELKALVLFLKNGADCPCHQLDNLSHNFLIMGRKVKSQYLLTAIHKWDKKNKEFKNFMKRMKNHECPTFQSVFK

Approximately 32.6 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.