1. 重组蛋白
  2. CD Antigens
  3. Macrophage CD Proteins Stem Cell CD Proteins Endothelial cell CD Proteins
  4. Thy-1/CD90
  5. Thy1/CD90 蛋白, Human (HEK293)

Thy1/CD90 蛋白, Human (HEK293)

目录号: HY-P74518
COA 产品使用指南

THY1 膜糖蛋白 (THY1, CD90) 是一种细胞表面糖蛋白,是免疫球蛋白超家族的成员。THY1 参与细胞粘附和细胞通讯,在免疫和神经系统细胞中尤为重要。THY1 可能在鼻咽癌中作为肿瘤抑制因子发挥作用,并可能在突触发生和大脑其他事件中的细胞-细胞或细胞-配体相互作用中发挥作用。Thy1/CD90 蛋白, Human (HEK293) 是重组的 Thy1/CD90 蛋白,由 HEK293 表达,不带标签。Thy1/CD90 蛋白, Human (HEK293) 全长 111 个氨基酸,分子量约为 ~23-30 kDa。

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描述

Thy-1 membrane glycoprotein (THY1, CD90) is a cell surface glycoprotein and member of the immunoglobulin superfamily of proteins. THY1 is involved in cell adhesion and cell communication particularly in cells of the immune and nervous systems. THY1 may function as a tumor suppressor in nasopharyngeal carcinoma and may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. Thy1/CD90 Protein, Human (HEK293) is the recombinant human-derived Thy1/CD90 protein, expressed by HEK293 , with tag free. The total length of Thy1/CD90 Protein, Human (HEK293) is 111 a.a., with molecular weight of ~23-30 kDa.

研究背景

THY1 膜糖蛋白 (THY1, CD90) 是一种细胞表面糖蛋白,是免疫球蛋白超家族的成员。在许多细胞类型中,THY1 参与细胞粘附和细胞通讯,在免疫和神经系统细胞中尤为重要。THY1 被广泛用作造血干细胞的标记物。THY1 可能在鼻咽癌中作为肿瘤抑制因子发挥作用,并可能在突触发生和大脑其他事件中的细胞-细胞或细胞-配体相互作用中发挥作用,促进细胞之间的交流和连接并塑造神经网络的结构和功能[1][2]

种属

Human

表达系统

HEK293

标签

Tag Free

蛋白编号

P04216/NP_006279.2(Q20-C130)

基因 ID
蛋白结构
N-term
Thy1 (Q20-C130)
Accession # NP_006279.2
C-term
同用名
Thy-1 membrane glycoprotein; CDw90; Thy-1 antigen; CD90
氨基酸序列

QKVTSLTACLVDQSLRLDCRHENTSSSPIQYEFSLTRETKKHVLFGTVGVPEHTYRSRTNFTSKYNMKVLYLSAFTSKDEGTYTCALHHSGHSPPISSQNVTVLRDKLVKC

分子量

Approximately 23-30 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Thy1/CD90 Protein, Human (HEK293)
目录号:
HY-P74518
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