TIM-4/TIMD-4 蛋白, Mouse (HEK293, His)

The TIM-4/TIMD-4 protein is a phosphatidylserine receptor that regulates immune responses through multiple actions. It participates in phagocytosis of apoptotic cells and regulates T cell function. TIM-4/TIMD-4 Protein, Mouse (HEK293, His) is the recombinant mouse-derived TIM-4/TIMD-4 protein, expressed by HEK293 , with C-His labeled tag.

TIM-4/TIMD-4 蛋白作为磷脂酰丝氨酸受体，在免疫反应中发挥多种作用，参与凋亡细胞的吞噬作用和 T 细胞功能的调节。它对 T 细胞激活的参与是双模式的，因为它通过诱导细胞周期停滞来减少初始 T 细胞的激活，同时通过激活 AKT1 和 ERK1/2 磷酸化以及随后的信号通路来增强激活 T 细胞的增殖。此外，TIM-4/TIMD-4 通过直接与凋亡细胞上的磷脂酰丝氨酸结合，从而促进其被吞噬细胞吞噬，从而在胞吞作用（凋亡细胞的吞噬过程）中发挥关键作用。这种功能是独特的，因为其他吞噬受体（如 MERTK）在识别和介导凋亡细胞的摄取方面更有效。此外，TIM-4/TIMD-4 通过在磷脂酰丝氨酸依赖性机制中激活 STK11/PRKAA1 通路来抑制 NLRP3 炎性体活性，从而促进自噬过程。该蛋白质形成同二聚体，在免疫调节中发挥多方面的作用。

Mouse

HEK293

C-6*His

Q6U7R4/NP_848874.3 (A23-T279)

276891  [NCBI]

ASEDTIIGFLGQPVTLPCHYLSWSQSRNSMCWGKGSCPNSKCNAELLRTDGTRIISRKSTKYTLLGKVQFGEVSLTISNTNRGDSGVYCCRIEVPGWFNDVKKNVRLELRRATTTKKPTTTTRPTTTPYVTTTTPELLPTTVMTTSVLPTTTPPQTLATTAFSTAVTTCPSTTPGSFSQETTKGSAFTTESETLPASNHSQRSMMTISTDIAVLRPTGSNPGILPSTSQLTTQKTTLTTSESLQKTTKSHQINSRQT

Approximately 60-85 kDa due to the glycosylation.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.