Neurotensin(8-13) (Synonyms: 神经降压素(8-13))

目录号: HY-P0251 纯度: 99.95%: FAQs
Data Sheet SDS COA 产品使用指南

摩尔计算器

Neurotensin (8-13) 是神经降压素的活性片段，Neurotensin (8-13) 降低细胞表面NT1受体 (NTR1) 密度。

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Custom Peptide Synthesis

Neurotensin(8-13) Chemical Structure

CAS No. : 60482-95-3

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规格	价格	是否有货
1 mg	￥600	In-stock
5 mg	￥1200	In-stock
10 mg	￥2100	In-stock
25 mg	￥4400	In-stock
50 mg		询价
100 mg		询价

or 大包装询价

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Customer Review

Other Forms of Neurotensin(8-13):

Neurotensin(8-13) TFA 询价

MCE 顾客使用本产品发表的 1 篇科研文献

•Nat Struct Mol Biol. 2022 Mar;29(3):210-217. [Abstract]

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NTR1 NTR2

生物活性
实验参考方法
纯度 & 产品资料
参考文献

生物活性

Neurotensin (8-13) is an active fragment of Neurotensin. Neurotensin(8-13) results in a decrease in cell-surface NT1 receptors (NTR1) density.

IC₅₀ & Target

NTR1

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
CHO	EC₅₀	4.16 x 10^-5 μM Compound: NT(8-13)	Agonist activity at NTSR1 (unknown origin) expressed in CHO cells assessed as potentiation of NT(8-13) peptide-induced change in intracellular Ca2+ level preincubated for 45 mins by FLIPR assay Agonist activity at NTSR1 (unknown origin) expressed in CHO cells assessed as potentiation of NT(8-13) peptide-induced change in intracellular Ca2+ level preincubated for 45 mins by FLIPR assay	[PMID: 24997685]
CHO-K1	IC₅₀	0.82 nM Compound: NT[8-13]	Displacement of [125I]-Tyr3-NT from human NTS1 receptor expressed in CHOK1 cell membranes incubated for 30 mins by gamma-counting based competitive radioligand binding assay Displacement of [125I]-Tyr3-NT from human NTS1 receptor expressed in CHOK1 cell membranes incubated for 30 mins by gamma-counting based competitive radioligand binding assay	[PMID: 26348111]
CHO-K1	EC₅₀	1.42 nM Compound: NT[8-13]	Agonist activity at human NTS1 receptor expressed in CHOK1 cells co-expressing Galphaq-RlucII(121)/Gbeta1/GFP10-Ggamma1 assessed as Galpha-q stimulation incubated for 15 mins by BRET assay Agonist activity at human NTS1 receptor expressed in CHOK1 cells co-expressing Galphaq-RlucII(121)/Gbeta1/GFP10-Ggamma1 assessed as Galpha-q stimulation incubated for 15 mins by BRET assay	[PMID: 26348111]
CHO-K1	EC₅₀	1.85 nM Compound: NT[8-13]	Agonist activity at human NTS1 receptor expressed in CHOK1 cells co-expressing hNTS1-GFP10/RlucII-beta-arrestin 2 assessed as beta-arrestin2 recruitment incubated for 15 mins by BRET assay Agonist activity at human NTS1 receptor expressed in CHOK1 cells co-expressing hNTS1-GFP10/RlucII-beta-arrestin 2 assessed as beta-arrestin2 recruitment incubated for 15 mins by BRET assay	[PMID: 26348111]
HT-29	EC₅₀	1.4 nM Compound: NT(8-13)	Agonist activity at NTSR1 in human HT-29 cells assessed as induction of intracellular Ca2+ mobilization by fura-2 dye-based spectrofluorimetric analysis Agonist activity at NTSR1 in human HT-29 cells assessed as induction of intracellular Ca2+ mobilization by fura-2 dye-based spectrofluorimetric analysis	[PMID: 26824643]

体外研究
(In Vitro)

Neurotensin (8-13) 诱导的受体内化导致细胞表面 NT1 受体 (NTR1) 密度降低。已针对 HT-29 细胞和大鼠原代培养神经元中的神经降压素 (NT) 描述了响应高胞外肽浓度的受体下调。细胞表面受体的再现也不同^[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

816.99

Formula

C₃₈H₆₄N₁₂O₈

CAS 号

60482-95-3

性状

固体

颜色

White to off-white

Sequence

Arg-Arg-Pro-Tyr-Ile-Leu

Sequence Shortening

RRPYIL

中文名称

神经降压素(8-13)

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture and light

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶解性数据

细胞实验：

H₂O 中的溶解度 : 50 mg/mL (61.20 mM; 超声助溶)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	1.2240 mL	6.1200 mL	12.2401 mL
5 mM	0.2448 mL	1.2240 mL	2.4480 mL
10 mM	0.1224 mL	0.6120 mL	1.2240 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物实验：

以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

方案一

请依序添加每种溶剂： PBS
Solubility: 33.33 mg/mL (40.80 mM); 澄清溶液; 超声助溶

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

计算结果

工作液所需浓度 : mg/mL

该产品水溶性佳，请具体参考实测水 / PBS / Saline 中的溶解度数据。

您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。

纯度 & 产品资料

纯度: 99.95%

选择批次：

Data Sheet (622 KB) SDS (393 KB)

COA (267 KB) RP-HPLC (277 KB)

产品使用指南 (1538 KB)

参考文献

[1]. García-Garayoa E, et al. Preclinical evaluation of a new, stabilized neurotensin(8--13) pseudopeptide radiolabeled with (99m)tc. J Nucl Med. 2002 Mar;43(3):374-83. [Content Brief]

Kinase Assay ^[1]	Binding assays are performed on whole HT-29 cells at confluence. A day before the assay, cells (10⁶ cells/0.4 mL, equivalent to 0.3 mg protein) are placed in 48-well plates. A special binding buffer that includes protease inhibitors (50 mM HEPES, 125 mM NaCl, 7.5 mM KCl, 5.5 mM MgCl₂, 1 mM EGTA, 5 g/L bovine serum albumin, 2 mg/L chymostatin, 100 mg/L soybean trypsin inhibitor, 50 mg/L bacitracin, pH 7.4) is used for the experiments. In inhibition studies, cells are incubated for 1 h at 37°C in triplicate with 25,000 cpm of ¹²⁵I-NT and variable concentrations (0.001-3,000 nM) of unlabeled NT(8-13), unlabeled NT-VIII, or NT-VIII labeled with ^natRe (final volume of 0.2 mL per well). The cells are then washed twice with cold binding buffer and afterward are solubilized with 1N NaOH at 37°C (0.4 mL per well). The activity is determined in a γ-counter. In saturation studies, cells are incubated in triplicate with increasing concentrations (0.1-10 nM) of ^99mTc(CO)3NT-VIII for 1 h at 37°C (final volume, 0.2 mL per well). The concentrations of total technetium (^99+99mTc) are equivalent to 0.2-20 MBq ^99mTc activity per well. After 2 washings with the same binding buffer as before, the cells are then solubilized with 1N NaOH at 37°C (0.4 mL per well). The bound radioactivity is measured in the γ-counter. Nonspecific binding is determined with 1 μM unlabeled NT(8-13)^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. García-Garayoa E, et al. Preclinical evaluation of a new, stabilized neurotensin(8--13) pseudopeptide radiolabeled with (99m)tc. J Nucl Med. 2002 Mar;43(3):374-83. [Content Brief]

Kinase Assay
^[1]

Binding assays are performed on whole HT-29 cells at confluence. A day before the assay, cells (10⁶ cells/0.4 mL, equivalent to 0.3 mg protein) are placed in 48-well plates. A special binding buffer that includes protease inhibitors (50 mM HEPES, 125 mM NaCl, 7.5 mM KCl, 5.5 mM MgCl₂, 1 mM EGTA, 5 g/L bovine serum albumin, 2 mg/L chymostatin, 100 mg/L soybean trypsin inhibitor, 50 mg/L bacitracin, pH 7.4) is used for the experiments. In inhibition studies, cells are incubated for 1 h at 37°C in triplicate with 25,000 cpm of ¹²⁵I-NT and variable concentrations (0.001-3,000 nM) of unlabeled NT(8-13), unlabeled NT-VIII, or NT-VIII labeled with ^natRe (final volume of 0.2 mL per well). The cells are then washed twice with cold binding buffer and afterward are solubilized with 1N NaOH at 37°C (0.4 mL per well). The activity is determined in a γ-counter. In saturation studies, cells are incubated in triplicate with increasing concentrations (0.1-10 nM) of ^99mTc(CO)3NT-VIII for 1 h at 37°C (final volume, 0.2 mL per well). The concentrations of total technetium (^99+99mTc) are equivalent to 0.2-20 MBq ^99mTc activity per well. After 2 washings with the same binding buffer as before, the cells are then solubilized with 1N NaOH at 37°C (0.4 mL per well). The bound radioactivity is measured in the γ-counter. Nonspecific binding is determined with 1 μM unlabeled NT(8-13)^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

[1]. García-Garayoa E, et al. Preclinical evaluation of a new, stabilized neurotensin(8--13) pseudopeptide radiolabeled with (99m)tc. J Nucl Med. 2002 Mar;43(3):374-83. [Content Brief]

Neurotensin(8-13) 相关分类

Neurological Disease

完整储备液配制表

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	1.2240 mL	6.1200 mL	12.2401 mL	30.6001 mL
	5 mM	0.2448 mL	1.2240 mL	2.4480 mL	6.1200 mL
	10 mM	0.1224 mL	0.6120 mL	1.2240 mL	3.0600 mL
	15 mM	0.0816 mL	0.4080 mL	0.8160 mL	2.0400 mL
	20 mM	0.0612 mL	0.3060 mL	0.6120 mL	1.5300 mL
	25 mM	0.0490 mL	0.2448 mL	0.4896 mL	1.2240 mL
	30 mM	0.0408 mL	0.2040 mL	0.4080 mL	1.0200 mL
	40 mM	0.0306 mL	0.1530 mL	0.3060 mL	0.7650 mL
	50 mM	0.0245 mL	0.1224 mL	0.2448 mL	0.6120 mL
	60 mM	0.0204 mL	0.1020 mL	0.2040 mL	0.5100 mL

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Neurotensin(8-13)60482-95-3神经降压素(8-13)Neurotensin ReceptorInhibitorinhibitorinhibit

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MedChemExpress (MCE) 只为有资质的科研机构、医药企业基于科学研究或药证申报的用途提供医药研发服务，不为任何个人或者非科研性质的、非用于药证申报使用等其他用途提供服务。

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沪(浦)应急管危经许[2021]201709(QFYS)

营业执照（三证合一）

Neurotensin(8-13) (Synonyms: 神经降压素(8-13))

Customer Review

Other Forms of Neurotensin(8-13):

MCE 顾客使用本产品发表的 1 篇科研文献