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  3. Ninhydrin-sodium molybdate chromogenic analytical probe for the assay of amino acids and proteins

Ninhydrin-sodium molybdate chromogenic analytical probe for the assay of amino acids and proteins

  • Spectrochim Acta A Mol Biomol Spectrosc. 2017 Feb 15;173:897-903. doi: 10.1016/j.saa.2016.10.040.
Shivakumar Anantharaman 1,  Nagaraja Padmarajaiah 2,  Naef Ghllab Saeed Al-Tayar 3,  Ashwinee Kumar Shrestha 4
Affiliations

Affiliations

  • 1 PG Department of Chemistry, St. Philomena's College, Bannimantap, Mysore 570015, India. Electronic address: shivakem77@yahoo.co.in.
  • 2 DOS in Chemistry, Manasagangotri, Mysore 570 006, India.
  • 3 Thamar University, Faculty of Applied Science, Department of Chemistry, Thamar, Yemen.
  • 4 Department of Pharmacy, Kathmandu University, Nepal.
PMID: 27821372 DOI: 10.1016/j.saa.2016.10.040
Abstract

A sensitive method has been proposed for the quantification of Amino acids and proteins using ninhydrin and sodium molybdate as chromogenic substrates in citrate buffer of pH5.6. A weak molybdate-hydrindantin complex plays the role in the formation of Ruhemann's purple. The linear response for the amino acid, amino acid mixture and Bovine serum albumin is between 0.999 and 66.80μM, 1.52 and 38μM and 5 and 100μg/L, respectively. The molar absorptivity of the individual amino acid by the proposed reaction extends from 0.58×104 to 2.86×104M-1cm-1. The linearity equations for the proposed ninhydrin-molybdate for amino acid mixture is Abs=0.021×Conc (μM)-0.002. The applicability of the proposed method has been justified in food and biological samples in conjunction with Kjeldahl method.

Keywords

Amino acid; Hydrindantin; Kjeldahl nitrogen; Leucine equivalents; Ninhydrin; Ruhemann's purple; Sodium molybdate.

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