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  3. The ZAR1 resistosome is a calcium-permeable channel triggering plant immune signaling

The ZAR1 resistosome is a calcium-permeable channel triggering plant immune signaling

  • Cell. 2021 Jun 24;184(13):3528-3541.e12. doi: 10.1016/j.cell.2021.05.003.
Guozhi Bi 1,  Min Su 2,  Nan Li 2,  Yu Liang 3,  Song Dang 2,  Jiachao Xu 2,  Meijuan Hu 1,  Jizong Wang 4,  Minxia Zou 5,  Yanan Deng 2,  Qiyu Li 2,  Shijia Huang 6,  Jiejie Li 5,  Jijie Chai 7,  Kangmin He 8,  Yu-Hang Chen 9,  Jian-Min Zhou 10
Affiliations

Affiliations

  • 1 State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
  • 2 State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
  • 3 State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China.
  • 4 State Key Laboratory of Protein and Plant Gene Research, School of Advanced Agricultural Sciences, Peking University, Beijing 100871, China.
  • 5 Beijing Key Laboratory of Gene Resource and Molecular Development, College of Life Science, Beijing Normal University, Beijing 100875, China; Key Laboratory of Cell Proliferation and Regulation of Ministry of Education, College of Life Science, Beijing Normal University, Beijing 100875, China.
  • 6 Beijing Advanced Innovation Center for Structural Biology, Tsinghua-Peking Joint Center for Life Sciences, Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.
  • 7 Beijing Advanced Innovation Center for Structural Biology, Tsinghua-Peking Joint Center for Life Sciences, Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China; Max-Planck Institute for Plant Breeding Research, Cologne, Germany; Institute of Biochemistry, University of Cologne, Zuelpicher Str. 47, 50674 Cologne, Germany. Electronic address: chaijj@tsinghua.edu.cn.
  • 8 State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China; Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing 100101, China. Electronic address: kmhe@genetics.ac.cn.
  • 9 State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China; Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing 100101, China. Electronic address: yuhang.chen@genetics.ac.cn.
  • 10 State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing 100101, China; CAS Center for Excellence in Biotic Interactions, University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: jmzhou@genetics.ac.cn.
PMID: 33984278 DOI: 10.1016/j.cell.2021.05.003
Abstract

Nucleotide-binding, leucine-rich repeat receptors (NLRs) are major immune receptors in Plants and Animals. Upon activation, the Arabidopsis NLR protein ZAR1 forms a pentameric resistosome in vitro and triggers immune responses and cell death in Plants. In this study, we employed single-molecule imaging to show that the activated ZAR1 protein can form pentameric complexes in the plasma membrane. The ZAR1 resistosome displayed ion channel activity in Xenopus oocytes in a manner dependent on a conserved acidic residue Glu11 situated in the channel pore. Pre-assembled ZAR1 resistosome was readily incorporated into planar lipid-bilayers and displayed calcium-permeable cation-selective channel activity. Furthermore, we show that activation of ZAR1 in the plant cell led to Glu11-dependent CA2+ influx, perturbation of subcellular structures, production of Reactive Oxygen Species, and cell death. The results thus support that the ZAR1 resistosome acts as a calcium-permeable cation channel to trigger immunity and cell death.

Keywords

Arabidopsis; NLR; Pseudomonas; Xanthomonas; calcium channel; cell death; disease resistance; plant immunity; resistosome; single-molecule imaging.

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