2. Academic Validation
  3. Rapid metabolic reprogramming mediated by the AMP-activated protein kinase during the lytic cycle of Toxoplasma gondii

Rapid metabolic reprogramming mediated by the AMP-activated protein kinase during the lytic cycle of Toxoplasma gondii

  • Nat Commun. 2023 Jan 26;14(1):422. doi: 10.1038/s41467-023-36084-0.
Yaqiong Li 1,  Zhipeng Niu # 1,  Jichao Yang # 1,  Xuke Yang # 1,  Yukun Chen 1,  Yingying Li 1,  Xiaohan Liang 1,  Jingwen Zhang 1,  Fuqiang Fan 1,  Ping Wu 2,  Chao Peng 2,  Bang Shen 3 4 5
Affiliations

Affiliations

  • 1 State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei Province, 430070, PR China.
  • 2 National Facility for Protein Science in Shanghai, Zhangjiang Lab, Shanghai Advanced Research Institute, Chinese Academy of Science, Shanghai, 201210, PR China.
  • 3 State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei Province, 430070, PR China. shenbang@mail.hzau.edu.cn.
  • 4 Key Laboratory of Preventive Medicine in Hubei Province, Wuhan, Hubei Province, 430070, PR China. shenbang@mail.hzau.edu.cn.
  • 5 Hubei Hongshan Laboratory, Wuhan, Hubei Province, 430070, PR China. shenbang@mail.hzau.edu.cn.
  • # Contributed equally.
PMID: 36702847 DOI: 10.1038/s41467-023-36084-0
Abstract

The ubiquitous pathogen Toxoplasma gondii has a complex lifestyle with different metabolic activities at different stages that are intimately linked to the parasitic environments. Here we identified the eukaryotic regulator of cellular homeostasis AMP-activated protein kinase (AMPK) in Toxoplasma and discovered its role in metabolic programming during parasite's lytic cycle. The catalytic subunit AMPKα is quickly phosphorylated after the release of intracellular parasites to extracellular environments, driving energy-producing catabolism to power Parasite motility and invasion into host cells. Once inside host cells, AMPKα phosphorylation is reduced to basal level to promote a balance between energy production and biomass synthesis, allowing robust Parasite replication. AMPKγ depletion abolishes AMPKα phosphorylation and suppresses Parasite growth, which can be partially rescued by overexpressing wildtype AMPKα but not the phosphorylation mutants. Thus, through the cyclic reprogramming by AMPK, the parasites' metabolic needs at each stage are satisfied and the lytic cycle progresses robustly.

Figures
Products
嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z