Baicalin promotes random-pattern skin flap survival by inducing autophagy via AMPK-regulated TFEB nuclear transcription

The random-pattern skin FLAP is a generally used technique to cover the soft tissue defect, while its application is often constrained by complications after the FLAP transplant. Necrosis of the FLAP remains a principal obstacle. The purpose of this study was to investigate the effect of Baicalin on skin FLAP survival and its mechanism. First of all, we discovered that administering Baicalin stimulated cell migration and boosted the formation of capillary tubes in human umbilical vein endothelial cells. Then, we detected that Baicalin reduced apoptosis-induced oxidative stress by using western blot and oxidative stress test kit. After that, we observed that Baicalin increased Autophagy and utilized 3MA to block Autophagy augmentation substantially reversing the effects of Baicalin therapy. Furthermore, we uncovered the underlying mechanisms of Baicalin-induced Autophagy via AMPK-regulated TFEB nuclear transcription. Finally, our in vivo experiment findings showed that Baicalin reduces oxidative stress, inhibits Apoptosis, promotes angiogenesis, and boosts the levels of Autophagy. After Autophagy was blocked, substantially reversing the effects of Baicalin therapy. Our study indicated that Baicalin-induced Autophagy via AMPK regulated TFEB nuclear transcription and then promotes angiogenesis and against oxidative stress and apoptotic promotes skin FLAP survival. These findings highlight the therapeutic potential for the clinical application of Baicalin in the future.

AMPK-TFEB; Baicalin; apoptosis; autophagy; random-pattern skin flap.