2. PI3K/Akt/mTOR Autophagy Metabolic Enzyme/Protease
  3. PI3K Autophagy Mitophagy Endogenous Metabolite
  4. 3-Methyladenine

3-Methyladenine  (Synonyms: 3-甲基腺嘌呤; 3-MA)

目录号: HY-19312 纯度: 99.91%
COA 产品使用指南

摩尔计算器

3-Methyladenine (3-MA) 是 PI3K 的抑制剂。它通过抑制class III PI3K广泛作为自噬 (autophagy) 的抑制剂使用。

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3-Methyladenine Chemical Structure

3-Methyladenine Chemical Structure

CAS No. : 5142-23-4

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Customer Review

Other Forms of 3-Methyladenine:

3-Methyladenine 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 778 篇科研文献

WB
IF

    3-Methyladenine purchased from MCE. Usage Cited in: J Nanobiotechnology. 2023 Feb 28;21(1):69.  [Abstract]

    The autophagy is inhibited by 3-Methyladenine (3-MA; 5 mM; 24 h), as evidenced by decreased Atg5 expression and the ratio of LC3-II/LC3-I and increased p62 protein level in MLE-12 cells (Fig. B, C).

    3-Methyladenine purchased from MCE. Usage Cited in: J Nanobiotechnology. 2023 Feb 28;21(1):69.  [Abstract]

    3-Methyladenine (3-MA; 5 mM; 24 h) markedly decreases the SWCNTs-induced increase in LC3B puncta, in MLE-12 cells.

    3-Methyladenine purchased from MCE. Usage Cited in: Brain Behav Immun. 2019 Jul;79:244-255.  [Abstract]

    Immunoblot analysis of NLRP3 in LPS-primed macrophages treated with KA (10 μM) or concurrently with autophagy inhibitor (3-MA, 5 mM). Statistical analysis of NLRP3 intensity during 3-MA or zaprinast treatment is shown.

    3-Methyladenine purchased from MCE. Usage Cited in: Ecotoxicol Environ Saf. 2019 Apr 15;170:112-119.  [Abstract]

    Western blotting image protein levels of GRP78/Bip in DF-1 cells treated with different supplements (+ represent supplying, - represents not supplying a certain amount of Cr(VI), 4-PBA, Tg, NS-398, 3-MA and Rapamycin).

    3-Methyladenine purchased from MCE. Usage Cited in: Ecotoxicol Environ Saf. 2019 Apr 15;170:112-119.  [Abstract]

    Western blotting image protein levels of LC3-I & LC3-II in DF-1 cells treated with different supplements (+ represent supplying, - represents not supplying a certain amount of Cr(VI), 4-PBA, Tg, NS-398, 3-MA and Rapamycin).

    3-Methyladenine purchased from MCE. Usage Cited in: Ecotoxicol Environ Saf. 2019 Apr 15;170:112-119.  [Abstract]

    Western blotting image protein levels of p62 in DF-1 cells treated with different supplements (+ represent supplying, - represents not supplying a certain amount of Cr(VI), 4-PBA, Tg, NS-398, 3-MA and Rapamycin).

    3-Methyladenine purchased from MCE. Usage Cited in: Front Endocrinol (Lausanne). 2019 Jun 17;10:370.  [Abstract]

    LC3 II expression is regulated in CQ- or 3-MA-treated GnRH-OE Panc1 cells.

    3-Methyladenine purchased from MCE. Usage Cited in: FASEB J. 2019 Apr;33(4):5520-5534.  [Abstract]

    CRC cells are treated with vehicle, BFA (100 nM), 3-MA (10 mM), or in combination for 24 h. Immunoblot analysis is used to detect LC3 turnover.

    3-Methyladenine purchased from MCE. Usage Cited in: J Cell Biochem. 2019 Mar;120(3):4366-4374.  [Abstract]

    Representative Western blots show LC3 I/II and p62 protein expression in kidneys of vehicle group and 3-MA group mice at 72 hours after the CDDP treatment.

    3-Methyladenine purchased from MCE. Usage Cited in: Int J Mol Med. 2018 Jun;41(6):3221-3230.  [Abstract]

    Western blot analysis is used to measure LC3, p62 and Beclin‑1 expression following the treatment of H9C2 cells with si‑miRNA‑30e and 3‑MA.

    3-Methyladenine purchased from MCE. Usage Cited in: Int J Mol Med. 2018 May;41(5):2535-2544.  [Abstract]

    MC3T3-E1 cells are treated with AICAR (10 μM) in the presence or absence of 3-MA (5 mM) or CQ (10 μM) for 24 h, after which western blot analysis is conducted.

    3-Methyladenine purchased from MCE. Usage Cited in: J Cell Mol Med. 2018 Oct;22(10):5132-5144.  [Abstract]

    Melatonin regulation of mitophagy activity in the diabetic myocardium, Protein expression with representative gel blots of LC3 I/II, GAPDH (loading control).

    3-Methyladenine purchased from MCE. Usage Cited in: Int J Oncol. 2018 Sep;53(3):1363-1373.  [Abstract]

    The protein expression levels of LC3 and Beclin-1 proteins in HCT-116 cells. Cells are treated with 100 μg/mL active fraction of clove (AFC) for 48 h with or without pretreatment of 2 mM 3-MA or 1 nM Baflomycin A1 (BA) for 1 h.

    3-Methyladenine purchased from MCE. Usage Cited in: J Dermatol Sci. 2018 Jan;89(1):11-18.  [Abstract]

    Inhibitory effects of LED 585 nm on melanogenesis is reversed by 3-MA. Autophagy is decreased in 3-MA-treated group, which is reflected by the decrease in the ratio of LC3 II/LC3 I and increase in p62 protein

    3-Methyladenine purchased from MCE. Usage Cited in: J Dermatol Sci. 2018 Jan;89(1):11-18.  [Abstract]

    Inhibitory effects of LED 585 nm on melanogenesis is reversed by 3-MA.

    3-Methyladenine purchased from MCE. Usage Cited in: J Immunol Res. 2018 Dec 24;2018:9807139.  [Abstract]

    Western blot assays show that 3-MA abrogates the Alda-1 induced expression increases in LC3BⅡ and Bcl2 and decreases in P62 and Bax.

    3-Methyladenine purchased from MCE. Usage Cited in: Acta Biochim Biophys Sin (Shanghai). 2018 Feb 1;50(2):144-155.  [Abstract]

    Autophagy is involved in Rg1-inhibited apoptosis in macrophages Macrophages with serum deprivation are treated with 50 μM Rg1 for 48 h in the absence or presence of 3-MA (5 mM). The protein expression level of cleaved caspase-3 determined by western blot analysis in Raw264.7 macrophages.

    3-Methyladenine purchased from MCE. Usage Cited in: PLoS One. 2018 Aug 6;13(8):e0201920.  [Abstract]

    The pretreatment of Z-VAD-FMK significantly reduces the levels of cleaved caspase-3 and cleaved PARP. The pretreatment of 3-MA blocks the conversion of LC3-I to LC3-II.

    3-Methyladenine purchased from MCE. Usage Cited in: Biomed Res. 2018;39(2):87-94.  [Abstract]

    Accumulation of LC3B II and p62 proteins in the livers of chloroform-treated mice is reduced by 3-MA treatment.

    3-Methyladenine purchased from MCE. Usage Cited in: Cell Physiol Biochem. 2018;48(6):2318-2336.  [Abstract]

    Cells are pretreated with 5 mM 3-MA or 2 μM for 1 h and then exposed to 40 μM EPA and 10 μM Rp for 48 h. Cell extracts are prepared and subjected to western blot analysis.

    3-Methyladenine purchased from MCE. Usage Cited in: Oncotarget. 2017 Nov 22;8(65):109135-109150.  [Abstract]

    Cell morphology of MCF-7 treated with RA (20 μM)+ω-3 PUFAs (80 μM) with or without 3-MA (5 mM) for 24h.

    3-Methyladenine purchased from MCE. Usage Cited in: Biochem Biophys Res Commun. 2016 Dec 2;481(1-2):90-96.  [Abstract]

    The hepatic stellate cells (HSCs) activation is down-regulated along with autophagy following DMKG or 3MA treatment in vitro. Western blot analysis of LC3B, Beclin-1, α-SMA, and collagen-I expression from lysates of HSC-T6 incubated with 4mM DMKG or 10 mM 3MA for 12 h.

    查看 PI3K 亚型特异性产品:

    查看所有亚型
    PI3Kα PI3Kβ PI3Kγ PI3Kδ PI3KC2α PI3KC2β PI3KC2γ Vps34 PI3K PI3KC3 p120γ

    • 生物活性

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    3-Methyladenine (3-MA) is a PI3K inhibitor. 3-Methyladenine is a widely used inhibitor of autophagy via its inhibitory effect on class III PI3K[1].

    IC50 & Target[1]

    PtdIns3Kγ

    60 μM (IC50, Cell Assay)

    Vps34

    25 μM (IC50, Cell Assay)

    Autophagy

     

    Mitophagy

     

    Human Endogenous Metabolite

     

    体外研究
    (In Vitro)

    3-Methyladenine (0-10 mM；0-48 小时) 以时间和剂量依赖性方式诱导 HeLa 细胞中半胱天冬酶依赖性细胞死亡[2]。
    3-Methyladenine (5 mM；24 小时) 抑制 HeLa 细胞在无葡萄糖条件和正常条件下的自噬[2]。
    3-Methyladenine (5 mM；0-48 小时) 抑制转化 LC3-I 与 LC3-II (自噬标记物) 在 12 小时和 48 小时之间的变化，证实了对自噬的抑制作用[2]。
    3-Methyladenine 诱导细胞死亡与自噬抑制无关[2]。
    3-Methyladenine 显著缩短了诺考达唑诱导的前中期停滞的持续时间[2]。
    注意：
    在细胞培养中，3-MA 的推荐浓度约为 0.5-10 mM。不推荐使用 DMSO 储备液。我们建议您在实验前称量您需要的 3-MA 的量，然后将其溶解在培养基中，并用 0.22 μm 过滤器除菌，现用现配。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    3-Methyladenine 相关抗体:

    Cell Viability Assay[2]

    Cell Line: HeLa cells
    Concentration: 0 mM, 2.5 mM or 5 mM, 10 mM
    Incubation Time: 0 hour, 24 hours and 48 hours
    Result: Decreased cell viability in a time-and dose-dependent manner, and was associated with caspase-3 activation.

    Cell Autophagy Assay[2]

    Cell Line: HeLa cells
    Concentration: 5 mM
    Incubation Time: 24 hours
    Result: Suppressed autophagy in HeLa cells under both glucose-free conditions and normal conditions.

    Western Blot Analysis[2]

    Cell Line: HeLa cells
    Concentration: 5 mM
    Incubation Time: 0 hour, 12 hours, 24 hours and 48 hours
    Result: Suppressed conversion of LC3-I to LC3-II between 12 hours and 48 hours.
    体内研究
    (In Vivo)

    3-Methyladenine (1.5 mg/100 g；腹膜内注射；3-24 小时) 处理可在 12 小时和 24 小时时减轻大鼠牛磺胆酸钠诱导的严重急性胰腺炎 (SAP)[3]。
    3-Methyladenine 抑制牛磺胆酸钠诱导的SAP胰腺腺泡细胞自噬[3]。
    3-Methyladenine 对PI3K/Akt信号通路和NF-牛磺胆酸钠诱导 SAP 中的 κB 信号通路[3]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: 10–12 weeks Specific pathogen free- (SPF-) grade healthy male Sprague-Dawley (SD) rats (250–290 g)[3]
    Dosage: 1.5 mg/100 g (1000 μM)
    Administration: Intraperitoneal injection
    Result: Alleviated Sodium Taurocholate-Induced SAP.
    分子量

    149.15

    Formula

    C6H7N5
    CAS 号
    性状

    固体

    颜色

    White to off-white

    中文名称

    3-甲基腺嘌呤
    结构分类
    初始来源
    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    溶解性数据
    细胞实验： 

    H2O 中的溶解度 : 5 mg/mL (33.52 mM; 超声助溶)

    DMSO 中的溶解度 : 8.33 mg/mL (55.85 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 6.7047 mL 33.5233 mL 67.0466 mL
    5 mM 1.3409 mL 6.7047 mL 13.4093 mL
    查看完整工作液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    动物实验：

    以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比； 如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

    • 方案 一

      请依序添加每种溶剂： PBS

      Solubility: 4 mg/mL (26.82 mM); 澄清溶液; 超声助溶

    • 方案 二

      请依序添加每种溶剂： 50% PEG300    50% Saline

      Solubility: 25 mg/mL (167.62 mM); 澄清溶液; 超声助溶 (<50°C)

    动物溶解方案计算器
    请输入动物实验的基本信息：

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    只
    由于实验过程有损耗，建议您多配一只动物的量
    计算结果
    工作液所需浓度 : mg/mL
    纯度 & 产品资料

    纯度: 99.91%

    COA (289 KB) HNMR (236 KB) RP-HPLC (253 KB) LCMS (94 KB) 元素分析报告 (213 KB)

    产品使用指南 (1538 KB)
    参考文献

    3-Methyladenine 相关分类

    完整工作液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 6.7047 mL 33.5233 mL 67.0466 mL 167.6165 mL
    5 mM 1.3409 mL 6.7047 mL 13.4093 mL 33.5233 mL
    10 mM 0.6705 mL 3.3523 mL 6.7047 mL 16.7617 mL
    15 mM 0.4470 mL 2.2349 mL 4.4698 mL 11.1744 mL
    20 mM 0.3352 mL 1.6762 mL 3.3523 mL 8.3808 mL
    25 mM 0.2682 mL 1.3409 mL 2.6819 mL 6.7047 mL
    30 mM 0.2235 mL 1.1174 mL 2.2349 mL 5.5872 mL
    DMSO 40 mM 0.1676 mL 0.8381 mL 1.6762 mL 4.1904 mL
    50 mM 0.1341 mL 0.6705 mL 1.3409 mL 3.3523 mL

    * 该产品常见使用浓度在 mM 级别；建议您称取所需的量，然后将其溶解在培养基中，并用 0.22 μm 过滤器除菌，现用现配。

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    3-Methyladenine5142-23-43-MA3-甲基腺嘌呤PI3KAutophagyMitophagyEndogenous MetabolitePhosphoinositide 3-kinaseMitochondrial AutophagyInhibitorinhibitorinhibit

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