2. Academic Validation
  3. NDV-induced autophagy enhances inflammation through NLRP3/Caspase-1 inflammasomes and the p38/MAPK pathway

NDV-induced autophagy enhances inflammation through NLRP3/Caspase-1 inflammasomes and the p38/MAPK pathway

  • Vet Res. 2023 Jun 5;54(1):43. doi: 10.1186/s13567-023-01174-w.
Juncheng Cai # 1 2 3 4,  Siyuan Wang # 1 2 3 4,  Haoyun Du 1 2 3 4,  Lei Fan 1 2 3 4,  WeiFeng Yuan 1 2 3 4,  Qiufan Xu 1 2 3 4,  Jinlian Ren 1 2 3 4,  Qiuyan Lin 1 2 3 4,  Bin Xiang 5,  Chan Ding 6,  Tao Ren 7 8 9 10,  Libin Chen 11 12 13 14
Affiliations

Affiliations

  • 1 College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China.
  • 2 National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, Guangzhou, China.
  • 3 Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, Guangzhou, China.
  • 4 Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, China.
  • 5 College of Veterinary Medicine, Yunnan Agricultural University, Kunming, China.
  • 6 Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, China.
  • 7 College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China. rentao6868@126.com.
  • 8 National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, Guangzhou, China. rentao6868@126.com.
  • 9 Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, Guangzhou, China. rentao6868@126.com.
  • 10 Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, China. rentao6868@126.com.
  • 11 College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China. chenlibin@scau.edu.cn.
  • 12 National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, Guangzhou, China. chenlibin@scau.edu.cn.
  • 13 Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, Guangzhou, China. chenlibin@scau.edu.cn.
  • 14 Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, China. chenlibin@scau.edu.cn.
  • # Contributed equally.
PMID: 37277829 DOI: 10.1186/s13567-023-01174-w
Abstract

Newcastle disease (ND), caused by the Newcastle disease virus (NDV), is a highly virulent infectious disease of poultry. Virulent NDV can cause severe Autophagy and inflammation in host cells. While studies have shown a mutual regulatory relationship between Autophagy and inflammation, this relationship in NDV Infection remains unclear. This study confirmed that NDV Infection could trigger Autophagy in DF-1 cells to promote cytopathic and viral replication. NDV-induced Autophagy was positively correlated with the mRNA levels of inflammatory cytokines such as IL-1β, IL-8, IL-18, CCL-5, and TNF-α, suggesting that NDV-induced Autophagy promotes the expression of inflammatory cytokines. Further investigation demonstrated that NLRP3 protein expression, Caspase-1 activity, and p38 phosphorylation level positively correlated with Autophagy, suggesting that NDV-induced Autophagy could promote the expression of inflammatory cytokines through NLRP3/Caspase-1 inflammasomes and p38/MAPK pathway. In addition, NDV Infection also triggered mitochondrial damage and Mitophagy in DF-1 cells, but did not result in a large leakage of Reactive Oxygen Species (ROS) and mitochondrial DNA (mtDNA), indicating that mitochondrial damage and Mitophagy do not contribute to the inflammation response during NDV Infection.

Keywords

Autophagy; Newcastle disease virus; inflammation; mitochondrial damage; mitophagy.

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