2. Academic Validation
  3. Vimentin binds to a novel tumor suppressor protein, GSPT1-238aa, encoded by circGSPT1 with a selective encoding priority to halt autophagy in gastric carcinoma

Vimentin binds to a novel tumor suppressor protein, GSPT1-238aa, encoded by circGSPT1 with a selective encoding priority to halt autophagy in gastric carcinoma

  • Cancer Lett. 2022 Oct 1;545:215826. doi: 10.1016/j.canlet.2022.215826.
Fan Hu 1,  Yin Peng 1,  Shanshan Chang 1,  Xiaonuan Luo 1,  Yuan Yuan 1,  Xiaohui Zhu 1,  Yidan Xu 1,  Kaining Du 1,  Yang Chen 1,  Shiqi Deng 1,  Fan Yu 1,  Xianling Feng 1,  Xinmin Fan 1,  Hassan Ashktorab 2,  Duane Smoot 3,  Stephen J Meltzer 4,  Song Li 5,  Yanjie Wei 6,  Xiaojing Zhang 7,  Zhe Jin 8
Affiliations

Affiliations

  • 1 Guangdong Provincial Key Laboratory of Genome Stability and Disease Prevention and Regional Immunity and Diseases, Department of Pathology, Shenzhen University School of Medicine, Shenzhen, Guangdong, 518060, PR China.
  • 2 Department of Medicine and Cancer Center, Howard University, College of Medicine, Washington, DC, 20060, USA.
  • 3 Department of Medicine, Meharry Medical Center, Nashville, TN, 37208, USA.
  • 4 Department of Medicine/GI Division, Johns Hopkins University School of Medicine and Sidney Ki-mel Comprehensive Cancer Center, Baltimore, MD, 21287, USA.
  • 5 Shenzhen Science & Technology Development Exchange Center, Shenzhen Science and Technology Building, Shenzhen, Guangdong, 518055, PR China.
  • 6 Center for High Performance Computing, Shenzhen Institutes of Advanced Technology, Shenzhen, Guangdong, 518000, PR China.
  • 7 Guangdong Provincial Key Laboratory of Genome Stability and Disease Prevention and Regional Immunity and Diseases, Department of Pathology, Shenzhen University School of Medicine, Shenzhen, Guangdong, 518060, PR China. Electronic address: apple432801@szu.edu.cn.
  • 8 Guangdong Provincial Key Laboratory of Genome Stability and Disease Prevention and Regional Immunity and Diseases, Department of Pathology, Shenzhen University School of Medicine, Shenzhen, Guangdong, 518060, PR China. Electronic address: zhejin@szu.edu.cn.
PMID: 35839920 DOI: 10.1016/j.canlet.2022.215826
Abstract

Circular RNAs (circRNAs) are covalently closed, endogenous molecules that are widespread in eukaryotes. Recent evidence indicates that circRNAs play important roles in carcinogenesis. Several circRNAs have been reported to comprise translatable RNA; however, whether circRNAs encode functional proteins remains unknown. In our study, circRNA Sequencing was carried out using five pathologically diagnosed gastric carcinoma (GC) samples and their paired adjacent normal tissues, we characterized the circRNA GSPT1 (circGSPT1), which is expressed at low levels in GC. Antibody detections, and mass spectrometry were used to validate active circRNA translation. The spanning junction open reading frame in circGSPT1, driven by an internal ribosome entry site (IRES), encodes a functional peptide, termed GSPT1-238aa. Interestingly, GSPT1-238aa tends to select the start codon used to initiate translation. This is the first finding of selective translation driven by IRES. CircGSPT1 and GSPT1-238aa halted the proliferation, migration, and invasion in GC cells in vitro. We also confirmed that the vimentin/Beclin1/14-3-3 complex interacts with GSPT1-238aa and modulates Autophagy via the PI3K/Akt/mTOR signaling pathway in GC cells. Our study reveals that GSPT1-238aa, a novel protein encoded by circGSPT1, halts GC tumorigenesis. We also provide insights into the function and underlying molecular mechanisms of GSPT1-238aa in GC and suggest that this protein represents a novel target for GC treatment.

Keywords

Autophagy; Circular RNAs; Gastric cancer; Translation; circGSPT1.

Figures
Products
嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z