ACKR1 蛋白, Human (Cell-Free, His)

ACKR1 is an atypical chemokine receptor that regulates chemokine levels and localization through high-affinity binding, induction of sequestration, degradation, or transcytosis. ACKR1, also known as a chemokine interceptor or decoy receptor, binds to chemokines such as CXCL11 and CXCL12/SDF1. ACKR1 Protein, Human (Cell-Free, His) is the recombinant human-derived ACKR1 protein, expressed by E. coli Cell-free , with N-10*His labeled tag.

ACKR1 是一种非典型趋化因子受体，通过与趋化因子高亲和力结合，充当趋化因子水平和定位的关键调节因子，导致趋化因子隔离、降解或转胞吞作用。ACKR1 也称为拦截器、趋化因子清除受体或趋化因子诱饵受体，充当 CXCL11 和 CXCL12/SDF1 等趋化因子的受体。与传统配体驱动的信号转导不同，趋化因子与 ACKR1 结合不会激活 G 蛋白介导的通路，而是诱导 β-arrestin 募集，导致配体内化并激活 MAPK 信号通路。ACKR1 在调节迁移中间神经元的 CXCR4 蛋白水平、调整其趋化因子反应性方面发挥着至关重要的作用。在神经胶质瘤细胞中，它通过 MEK/ERK 途径转导信号，有助于细胞生长和存活。虽然不参与正常造血祖细胞功能，但 ACKR1 在恶性造血细胞中被 CXCL11 激活，导致 ERK1/2 磷酸化，增强细胞粘附和迁移。此外，ACKR1 与 CXCR4 一起作为 HIV 分离株的有限子集的共受体，强调了其与微生物感染的参与。

Measured by its binding ability in a functional ELISA. Immobilized ACKR1 at 0.2 μg/well can bind human CCL2,the ED₅₀ of human CCL2 protein is ≤70 μg/ml.

Human

E. coli Cell-free

N-10*His

Q16570-1 (M1-S336)

2532  [NCBI]

MGNCLHRAELSPSTENSSQLDFEDVWNSSYGVNDSFPDGDYGANLEAAAPCHSCNLLDDSALPFFILTSVLGILASSTVLFMLFRPLFRWQLCPGWPVLAQLAVGSALFSIVVPVLAPGLGSTRSSALCSLGYCVWYGSAFAQALLLGCHASLGHRLGAGQVPGLTLGLTVGIWGVAALLTLPVTLASGASGGLCTLIYSTELKALQATHTVACLAIFVLLPLGLFGAKGLKKALGMGPGPWMNILWAWFIFWWPHGVVLGLDFLVRSKLLLLSTCLAQQALDLLLNLAEALAILHCVATPLLLALFCHQATRTLLPSLPLPEGWSSHLDTLGSKS

42 kDa

Greater than 85% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 0.15 M NaCl, 0.05% FOS12, 6% Trehalose,pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.