Angiopoietin-1 蛋白, Human (HEK293, Fc)

Angiopoietin-1 Protein, Human (HEK 293, Fc) is a secreted protein located on the outside of cell membranes and is an agonistic ligand of receptor tyrosine kinase that is mainly expressed by vascular endothelial cells and hematopoietic cells. Angiopoietin-1 Protein, Human (HEK 293, Fc) is a potent inducer of sprouting angiogenesis, proming for research of colorectal cancer liver metastases (CRCLMs). Angiopoietin-1 Protein, Human (HEK 293, Fc) induces vascular remodeling through highly organized angiogenesis and tightening of endothelial cell (EC) junctions. Angiopoietin-1 Protein, Human (HEK 293, Fc) regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and maintenance of vascular quiescence. Angiopoietin-1 Protein, Human (HEK 293, Fc) is a recombinant protein with a Fc label that consisting of 498 amino acids and is expressed in HEK293 cells^{[1][2][3][4][5][6][7]}.

Angiopoietin-1 蛋白, Human (HEK 293, Fc) 是一种分泌型蛋白配体，作用于“内膜内皮细胞激酶” Tek (Tie2)，主要在生长中的血管内皮细胞和一部分造血细胞中表达^[1]。
Angiopoietin-1 蛋白, Human (HEK 293, Fc) 通过血管 Tie2，整合素信号和非血管整合素信号诱导血管生成。Angiopoietin-1 是一种潜在的生长因子，可以用于治疗性血管生成和血管稳定^[1]。
Angiopoietin-1 蛋白, Human (HEK 293, Fc) 含有一个羧基末端的纤维蛋白原样结构域，能够与 Tie2 受体结合，还有一个中心的卷曲螺旋结构域，使这些纤维蛋白原样结构域能够聚合，以及一个短的氨基末端结构域，使聚合体聚集成不同大小的多聚体^[2]。
Angiopoietin-1 蛋白, Human (HEK 293, Fc) (0.5-10 nM) 在人脐静脉内皮细胞中以剂量依赖的方式诱导毛细血管芽的形成，并被可溶性 Tie2 受体胞外结构域完全抑制^[4]。
人和小鼠 Angiopoietin-1 蛋白的 cDNA 克隆显示，开放阅读框都编码 498 个氨基酸，共享 97.6% 的同源性^[5]。
Angiopoietin-1 蛋白, Human (HEK 293, Fc) 通过激活 JAK/STAT5 通路促进慢性髓性白血病中的 TKI 耐药性^[7]。

Angiopoietin-1 Protein, Human (HEK 293, Fc) (220 ng/mL) 以剂量依赖的方式与 VEGF ( 1 ng/mL) 协同引发毛细血管芽的形成，而这种形成在人脐静脉内皮细胞中被可溶性 Tie2 受体的外部结构完全抑制^[4]。
Angiopoietin-1 Protein, Human (HEK 293, Fc) (0-74 ng/mL) 诱导芽孢形成，但对人脐静脉内皮细胞的促生长活性却非常弱^[4]。
Angiopoietin-1 Protein, Human (HEK 293, Fc) 在人内皮细胞类型 (HUCEC, HAEC, HMVEC 和 HUVEC) 中诱导 TIE2 的酪氨酸磷酸化，这种诱导被添加过量的可溶性 TIE2-Fc 而不是 TrkB-Fc 所阻断^[5]。
Angiopoietin-1 Protein, Human (HEK 293, Fc) (0.2 μM, 24 小时) 通过 Tie2-PI3K/AKT 通路对 MC

Angiopoietin-1 Protein, Human (HEK 293, Fc) 的过度表达导致小鼠血管数量、大小和分支样式的急剧增加^[3]。
过度表达 Angiopoietin-1 Protein, Human (HEK 293, Fc) 的转基因小鼠在新生小鼠的皮肤上有更多的大血管，并且在老年小鼠的皮肤上比正常小鼠明显变得越来越红^[3]。

Measured by the ability of the immobilized protein to support the adhesion of HUVEC human umbilical vein endothelial cells. The ED₅₀ for this effect is ≤356.9 ng/ml, corresponding to a specific activity is ≥2801.905 units/mg.

• 
  Measured by the ability of the immobilized protein to support the adhesion of HUVEC human umbilical vein endothelial cells. The ED₅₀ for this effect is 356.9 ng/ml, corresponding to a specific activity is 2801.905 units/mg.

Human

HEK293

N-hFc

Q15389 (D256-F498)

284  [NCBI]

DTVHNLVNLCTKEGVLLKGGKREEEKPFRDCADVYQAGFNKSGIYTIYINNMPEPKKVFCNMDVNGGGWTVIQHREDGSLDFQRGWKEYKMGFGNPSGEYWLGNEFIFAITSQRQYMLRIELMDWEGNRAYSQYDRFHIGNEKQNYRLYLKGHTGTAGKQSSLILHGADFSTKDADNDNCMCKCALMLTGGWWFDACGPSNLNGMFYTAGQNHGKLNGIKWHYFKGPSYSLRSTTMMIRPLDF

Approximately 55-60 kDa due to the glycosylation.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween80 or PBS, pH 7.4, 8% trehalose or PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Gou Young Koh, et al. Orchestral actions of angiopoietin-1 in vascular regeneration. Trends Mol Med. 2013 Jan;19(1):31-9.  [Content Brief]

  [2]. Nuri Oh, et al. A Designed Angiopoietin-1 Variant, Dimeric CMP-Ang1 Activates Tie2 and Stimulates Angiogenesis and Vascular Stabilization in N-glycan Dependent Manner.Sci Rep. 2015 Oct 19;5:15291.  [Content Brief]

  [3]. Suri C, et al. Increased vascularization in mice overexpressing angiopoietin-1. Science. 1998 Oct 16;282(5388):468-71.  [Content Brief]

  [4]. Koblizek TI, et al. Angiopoietin-1 induces sprouting angiogenesis in vitro. Curr Biol. 1998 Apr 23;8(9):529-32.  [Content Brief]

  [5]. Davis S, et al. Isolation of angiopoietin-1, a ligand for the TIE2 receptor, by secretion-trap expression cloning. Cell. 1996 Dec 27;87(7):1161-9.  [Content Brief]

  [6]. Rada M, et al. Angiopoietin-1 Upregulates Cancer Cell Motility in Colorectal Cancer Liver Metastases through Actin-Related Protein 2/3. Cancers (Basel). 2022 May 21;14(10):2540.  [Content Brief]

  [7]. Ma D, et al. Intracellular angiopoietin-1 promotes TKI-resistance via activation of JAK/STAT5 pathway in chronic myeloid leukemia. Oncogene. 2023 Jan;42(2):124-137.  [Content Brief]