Angiopoietin-1 蛋白, Cynomolgus (HEK293, His)

The Angiopoietin-1 Protein is a secreted glycoprotein that is a key ligand in the vascular tyrosine kinase signaling pathway. ANGPT1 stimulates angiogenesis by activating PTK2/FAK and downstream kinase MAPK1/ERK2 and MAPK3/ERK1 signaling pathways. ANGPT1 affects the proliferation, migration and differentiation of skeletal muscle cells. Angiopoietin-1 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived Angiopoietin-1 protein, expressed by HEK293 , with N-His labeled tag.

血管生成素-1 (ANGPT1) 编码血管生成素家族中的一种分泌糖蛋白，是血管酪氨酸激酶信号通路的关键配体。ANGPT1 通过激活 PTK2/FAK 及下游激酶 MAPK1/ERK2、MAPK3/ERK1 信号通路刺激血管新生。Angiopoietin-1 Protein 调节血管的形成和稳定，在血管发育中有重要作用。ANGPT1 影响成骨骼肌细胞的增殖、迁移和分化。

Determined by its ability to induce adhesion of human umbilical vein endothelial cells (HUVEC).The ED₅₀ for this effect is 258.6 ng/mL, corresponding to a specific activity is 3.867×10³ units/mg.

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  Determined by its ability to induce adhesion of human umbilical vein endothelial cells (HUVEC).The ED₅₀ for this effect is 258.6 ng/mL, corresponding to a specific activity is 3.867×10³ units/mg.

Cynomolgus

HEK293

N-6*His

XP_005563980 (R276-F497)

102123994  [NCBI]

REEEKPFRDCADVYQAGFNKSGIYTIYINNMPEPKKVFCNMDVNGGGWTVIQHREDGSLDFQRGWKEYKMGFGNPSGEYWLGNEFIFAITSQRQYMLRIELMDWEGNRAYSQYDRFHIGNEKQNYRLYLKGHTGTAGKQSSLILHGADFSTKDADNDNCMCKCALMLTGGWWFDACGPSNLNGMFYTAGQNHGKLNGIKWHYFKGPSYSLRSTTMMIRPLDF

Approximately 30-34 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.