1. 重组蛋白
  2. Cytokines and Growth Factors Animal-free Recombinant Proteins
  3. VEGF & VEGFR
  4. VEGF
  5. VEGF-A
  6. Animal-Free VEGF165 蛋白, Human (His)

Animal-Free VEGF165 蛋白, Human (His)

目录号: HY-P700157AF
COA 产品使用指南

VEGF145 蛋白表达有限,表现出特殊的分布,并不广泛存在于组织中。它的有限出现意味着在生理过程中具有特定的、依赖于环境的作用。需要进一步的研究来揭示 VEGF145 积极参与的特定细胞环境和功能,从而深入了解其对局部生物活性的潜在贡献。Animal-Free VEGF165 蛋白, Human (His) 是重组的 无动物源 VEGF165 蛋白,由 E. coli 表达,带有 C-His 标签。Animal-Free VEGF165 蛋白, Human (His) 全长 165 个氨基酸,分子量约为 ~20.11 kDa。

无动物成分 (Animal-free) 重组蛋白,即生产过程中不使用或接触任何动物来源的材料和试剂,具有高一致性和稳定性。

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     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
Free Sample   Apply now  
2 μg ¥630 In-stock
10 μg ¥1750 In-stock
20 μg ¥2600 In-stock
50 μg ¥4900 In-stock
100 μg ¥8300 询价
> 100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

VEGF145 Protein, with limited expression, exhibits specialized distribution and is not broadly present in tissues. Its restricted occurrence implies a specific, context-dependent role in physiological processes. Further research is needed to unveil the specific cellular contexts and functions where VEGF145 actively participates, providing insights into its potential contributions to localized biological activities. Animal-Free VEGF165 Protein, Human (His) is the recombinant human-derived animal-FreeVEGF165 protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free VEGF165 Protein, Human (His) is 165 a.a., with molecular weight of ~20.11 kDa.

研究背景

VEGF145 蛋白的特点是表达有限,并未广泛分布于组织或细胞类型中。VEGF145 的有限出现表明其在生理过程中具有特殊且可能特定于环境的作用。需要进一步研究来揭示 VEGF145 积极参与的特定细胞环境和功能,从而揭示其对局部生物活性的潜在贡献。

生物活性

Measure by its ability to induce HUVEC cells proliferation. The ED50 for this effect is ≤5 ng/mL. The specific activity of recombinant human VEGF165 is approximately ≥1.4 x 106 IU/mg.

种属

Human

表达系统

E. coli

标签

C-His

蛋白编号

P15692-4 (A27-R191)

基因 ID
蛋白结构
N-term
VEGF165 (A27-R191)
Accession # P15692-4
His
C-term
同用名
rHuVEGF165; VPF; Folliculostellate cell-derived growth factor; Glioma-derived endothelial cell mitogen
氨基酸序列

MAPMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQENPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR

分子量

Approximately 20.11 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末

组分

Lyophilized from a solution containing 1X PBS, pH 8.0, trehalose.

内毒素含量

<0.1 EU per 1 μg of the protein by the LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Animal-Free VEGF165 Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Animal-Free VEGF165 Protein, Human (His)
目录号:
HY-P700157AF
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