1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. CAMKK1 蛋白, Human (Sf9, His)

CAMKK1 蛋白, Human (Sf9, His)

目录号: HY-P701723
产品使用指南

CAMKK1 蛋白是一种钙/钙调蛋白依赖性激酶,是参与各种细胞过程的钙触发信号级联的关键参与者。它磷酸化 CAMK1 家族的多个成员,包括 CAMK1、CAMK1D、CAMK1G 和 CAMK4。CAMKK1 蛋白, Human (Sf9, His) 是重组的 CAMKK1 蛋白,由 Sf9 insect cells 表达,带有 N-6*His 标签。CAMKK1 蛋白, Human (Sf9, His) 全长 505 个氨基酸。

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描述

The CAMKK1 protein is a calcium/calmodulin-dependent kinase and a key player in calcium-triggered signaling cascades involved in various cellular processes. It phosphorylates multiple members of the CAMK1 family, including CAMK1, CAMK1D, CAMK1G, and CAMK4. CAMKK1 Protein, Human (Sf9, His) is the recombinant human-derived CAMKK1 protein, expressed by Sf9 insect cells , with N-6*His labeled tag. The total length of CAMKK1 Protein, Human (Sf9, His) is 505 a.a., .

研究背景

CAMKK1 是一种钙/钙调蛋白依赖性蛋白激酶,在影响各种细胞过程的钙触发信号级联中发挥着关键作用。该激酶表现出磷酸化 CAMK1、CAMK1D、CAMK1G 和 CAMK4 的能力,表明其参与调节多个下游效应器。CAMKK1 尤其参与细胞凋亡的调节,它通过磷酸化 AKT1/PKB 来促进细胞存活。这种磷酸化事件反过来会抑制促凋亡蛋白 BAD(细胞死亡的 Bcl2 拮抗剂),凸显了 CAMKK1 在细胞生存途径中复杂的调节作用。

种属

Human

表达系统

Sf9 insect cells

标签

N-6*His

蛋白编号

Q8N5S9 (M1-S505)

基因 ID

84254

蛋白结构
N-term
6*His
CAMKK1 (M1-S505)
Accession # Q8N5S9
C-term
同用名
CAMKK1; Calcium/calmodulin-dependent protein kinase kinase 1; CaM-KK 1; CaM-kinase kinase 1; CaMKK 1; CaM-kinase IV kinase; Calcium/calmodulin-dependent protein kinase kinase alpha; CaM-KK alpha; CaM-kinase kinase alpha; CaMKK alpha
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

CAMKK1 Protein, Human (Sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CAMKK1 Protein, Human (Sf9, His)
目录号:
HY-P701723
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