1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. Cytosine deaminase/CodA 蛋白, E.coli (His-SUMO)

Cytosine deaminase/CodA 蛋白, E.coli (His-SUMO)

目录号: HY-P71466
产品使用指南

胞嘧啶脱氨酶 (CodA) 蛋白通过催化胞嘧啶水解脱氨基为尿嘧啶,在嘧啶补救途径中发挥关键作用。这种酶活性使细胞能够有效地利用胞嘧啶进行嘧啶核苷酸合成。Cytosine deaminase/CodA 蛋白, E.coli (His-SUMO) 是重组的 胞嘧啶脱氨酶/CodA 蛋白,由 E. coli 表达,带有 N-His, N-SUMO 标签。

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描述

Cytosine deaminase (CodA) protein plays a key role in the pyrimidine salvage pathway by catalyzing the hydrolytic deamination of cytosine to uracil. This enzyme activity enables cells to efficiently utilize cytosine for pyrimidine nucleotide synthesis. Cytosine deaminase/CodA Protein, E.coli (His-SUMO) is the recombinant E. coli-derived Cytosine deaminase/CodA protein, expressed by E. coli , with N-His, N-SUMO labeled tag.

研究背景

胞嘧啶脱氨酶由codA基因编码,是一种催化胞嘧啶水解脱氨基为尿嘧啶的酶。这种酶活性是嘧啶补救途径中的关键步骤,使细胞能够利用胞嘧啶合成嘧啶核苷酸。除了其主要功能外,胞嘧啶脱氨酶还对异鸟嘌呤(DNA 中腺嘌呤的氧化产物)和异胞嘧啶表现出脱氨酶活性。此外,该酶还表现出次级活性,将 5-氟胞嘧啶 (5FC) 转化为 5-氟尿嘧啶 (5FU)。这种将非细胞毒性前体 5FC 转化为细胞毒性化疗剂 5FU 的能力,凸显了胞嘧啶脱氨酶在癌症治疗中的潜在治疗应用。

种属

E.coli

表达系统

E. coli

标签

N-His;N-SUMO

蛋白编号

P25524 (S2-R427)

基因 ID

944966  [NCBI]

蛋白结构
N-term
6*His-SUMO
codA (S2-R427)
Accession # P25524
C-term
同用名
codA; b0337; JW0328Cytosine deaminase; CD; CDA; CDase; EC 3.5.4.1; Cytosine aminohydrolase; Isoguanine deaminase; EC 3.5.4.-
氨基酸序列

SNNALQTIINARLPGEEGLWQIHLQDGKISAIDAQSGVMPITENSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVKQRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGILSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDEIDDEQSRFVETVAALAHHEGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVANPLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHMGLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQVPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR

分子量

Approximately 63.5 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Cytosine deaminase/CodA Protein, E.coli (His-SUMO) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Cytosine deaminase/CodA Protein, E.coli (His-SUMO)
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HY-P71466
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