Cytosine deaminase/CodA 蛋白, E.coli (His-SUMO)

Cytosine deaminase (CodA) protein plays a key role in the pyrimidine salvage pathway by catalyzing the hydrolytic deamination of cytosine to uracil. This enzyme activity enables cells to efficiently utilize cytosine for pyrimidine nucleotide synthesis. Cytosine deaminase/CodA Protein, E.coli (His-SUMO) is the recombinant E. coli-derived Cytosine deaminase/CodA protein, expressed by E. coli , with N-His, N-SUMO labeled tag.

胞嘧啶脱氨酶由codA基因编码，是一种催化胞嘧啶水解脱氨基为尿嘧啶的酶。这种酶活性是嘧啶补救途径中的关键步骤，使细胞能够利用胞嘧啶合成嘧啶核苷酸。除了其主要功能外，胞嘧啶脱氨酶还对异鸟嘌呤（DNA 中腺嘌呤的氧化产物）和异胞嘧啶表现出脱氨酶活性。此外，该酶还表现出次级活性，将 5-氟胞嘧啶 (5FC) 转化为 5-氟尿嘧啶 (5FU)。这种将非细胞毒性前体 5FC 转化为细胞毒性化疗剂 5FU 的能力，凸显了胞嘧啶脱氨酶在癌症治疗中的潜在治疗应用。

E.coli

E. coli

N-His;N-SUMO

P25524 (S2-R427)

944966  [NCBI]

SNNALQTIINARLPGEEGLWQIHLQDGKISAIDAQSGVMPITENSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVKQRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGILSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDEIDDEQSRFVETVAALAHHEGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVANPLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHMGLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQVPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR

Approximately 63.5 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.