DDR1 蛋白, Human (HEK293, His)

The DDR1 protein is a tyrosine kinase and cell surface receptor for fibrillar collagen that tightly regulates cell attachment and affects extracellular matrix processes. Upon collagen binding, DDR1 initiates a signaling cascade involving SRC, activating MAP kinase. DDR1 Protein, Human (HEK293, His) is the recombinant human-derived DDR1 protein, expressed by HEK293 , with C-His labeled tag. The total length of DDR1 Protein, Human (HEK293, His) is 398 a.a., with molecular weight of 55-70 kDa.

DDR1 蛋白作为酪氨酸激酶并作为纤维胶原的细胞表面受体发挥作用，在调节细胞与细胞外基质的附着并影响细胞外基质重塑、细胞迁移、分化、存活和增殖等过程中发挥着关键作用。胶原蛋白结合后，DDR1 启动涉及 SRC 的信号级联并激活 MAP 激酶。这种激活反过来导致基质金属蛋白酶 MMP2、MMP7 和 MMP9 上调，促进细胞外基质重塑并促进细胞迁移和伤口愈合。DDR1的重要性延伸到各种生理过程，包括怀孕期间的正常囊胚着床、乳腺分化、哺乳以及耳朵形态和听力的维持。此外，DDR1 通过促进平滑肌细胞迁移来促进动脉伤口愈合，并在肿瘤细胞侵袭中发挥作用，进一步强调了其多样化的功能。值得注意的是，DDR1 磷酸化 PTPN11 作为其监管活动的一部分。

1. Immobilized Human DDR1, His Tag at 0.5 μg/mL (100 μl/well) on the plate. Dose response curve for Anti-DDR1 Antibody, hFc Tag with the EC₅₀ of <12.8 ng/mL determined by ELISA (QC Test).
2. Human DDR1, His Tag captured on CM5 Chip via Anti-His Antibody can bind Native Human Collagen I protein with an affinity constant of 0.806 nM as determined in SPR assay (Biacore T200).

Human

HEK293

C-His

Q08345 (D19-T416)

780  [NCBI]

55-70 kDa

Greater than 95% as determined by reducing SDS-PAGE or Tris-Bis PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.