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  3. Dit 蛋白, Bacillus phage SPP1 (FLAG, His)

Dit 蛋白, Bacillus phage SPP1 (FLAG, His)

目录号: HY-P701904
产品使用指南

Dit 蛋白是在其同源六聚体结构中在尾部远端形成 40 Å 宽通道的重要组成部分。这种独特的通道结构在细胞过程中发挥着至关重要的作用,并可能促进 DNA 的喷射。Dit 蛋白, Bacillus phage SPP1 (FLAG, His) 是重组的 Dit 蛋白,由 E. coli 表达,带有 N-6*His, N-Flag 标签。Dit 蛋白, Bacillus phage SPP1 (FLAG, His) 全长 140 个氨基酸。

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描述

The Dit protein is an important component that forms a 40 Å wide channel distal to the tail in its homohexameric structure. This unique channel structure plays a crucial role in cellular processes and may facilitate DNA ejection. Dit Protein, Bacillus phage SPP1 (FLAG, His) is the recombinant Dit protein, expressed by E. coli , with N-6*His, N-Flag labeled tag. The total length of Dit Protein, Bacillus phage SPP1 (FLAG, His) is 140 a.a., .

研究背景

Dit(远端尾蛋白)是一种同源六聚体蛋白,在病毒 DNA 喷射中发挥着至关重要的作用。它在病毒尾部的远端形成一个 40 埃宽的通道。值得注意的是,即使在 DNA 喷射过程之后,Dit 仍然与尾部结构相关。这意味着该蛋白质的结构特征有助于病毒尾部的稳定性和功能性。Dit 的同源六聚体性质表明了一种协调且有效的机制,可能有助于病毒生命周期中遗传物质的受控释放。

种属

Others

表达系统

E. coli

标签

N-6*His;N-Flag

蛋白编号

O48459 (N2-V141)

基因 ID

955334

蛋白结构
N-term
Flag-6*His
Dit (N2-V141)
Accession # O48459
C-term
同用名
Distal tail protein; Dit; Gene product 19.1; Gp19.1
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Dit Protein, Bacillus phage SPP1 (FLAG, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Dit Protein, Bacillus phage SPP1 (FLAG, His)
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HY-P701904
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