DR6/TNFRSF21 蛋白, Cynomolgus (HEK293, His)

Tnfrsf21 Protein, also known as death receptor 6 (DR6), CD358, or BM-018, is highly expressed in differentiating neurons as well as in the adult brain, and is upregulated in injured neurons. DR6 negatively regulates neuron, axon, and oligodendrocyte survival, hinders axondendrocyte and oligodendrocyte regeneration and its inhibition has a neuro-protective effect in nerve injury. It activates nuclear factor kappa-B (NFkB) and mitogen-activated protein kinase 8 (MAPK8, also called c-Jun N-terminal kinase 1), and induces cell apoptosis by associating with TNFRSF1A-associated via death domain (TRADD), which is known to mediate signal transduction of tumor necrosis factor receptors. DR6/TNFRSF21 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived DR6/TNFRSF21 protein, expressed by HEK293 , with C-His labeled tag. The total length of DR6/TNFRSF21 Protein, Cynomolgus (HEK293, His) is 309 a.a., with molecular weight of ~55-75 KDa.

Tnfrsf21 Protein 可能通过NF-kappa-B激活的途径促进细胞凋亡，并可通过BAX和细胞色素c从线粒体释放到细胞质中来促进细胞凋亡。Tnfrsf21 Protein 在神经元凋亡中发挥作用，包括对 APP 衍生的淀粉样肽的凋亡反应，并且是正常细胞体死亡和轴突修剪所必需的。Tnfrsf21 Protein 也作为焦亡的调节因子:在响应活性氧 (ROS) 和随后的氧化时招募 CASP8，导致 GSDMC 的激活。

Measured by its binding ability in a functional ELISA. When recombinant human APP770 is coated at 2 μg/mL (100 μL/well) can bind Recombinant Cynomolgus DR6. The ED₅₀ for this effect is 368.4 ng/mL.

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  Measured by its binding ability in a functional ELISA. When recombinant human APP770 is coated at 2 μg/mL (100 μL/well) can bind Recombinant Cynomolgus DR6. The ED₅₀ for this effect is 368.4 ng/mL.

Cynomolgus

HEK293

C-His

XP_005552846 (Q42-L350)

101925746  [NCBI]

QPEQKASNLIGTYRHVDRATGQVLTCDKCPAGTYVSEHCTNTSLRVCSSCPVGTFTRHENGIEKCHDCSQPCPWPMIEKLPCAALTDRECTCPPGMFQSNATCAPHTVCPVGWGVRKKGTETEDVRCKQCARGTFSDVPSSVMKCKAYTDCLSQNLVVIKPGTKEADNVCGTLPSFSSSTSPSPGTAIFSRPEHMDSHEVPSSTYVPKGMNSTESNSSASVRPKVLSSIQEGTVPDNTSSARGKEDVNKTLPNLQVVNHQQGPHHRHILKLLPSMEATGGEKSSTPIKGPKRGHPRQNLHKHFDINEHL

Approximately 55-75 kDa.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.