EIF2AK2 蛋白, Human (P. pastoris, His)

The EIF2AK2 protein is an interferon-inducing kinase that initiates the innate immune response against viral infection. It phosphorylates eIF-2-α, activating the integrated stress response, inhibiting overall protein synthesis and favoring ISR-specific mRNAs such as ATF4. EIF2AK2 Protein, Human (P. pastoris, His) is the recombinant human-derived EIF2AK2 protein, expressed by P. pastoris , with N-6*His labeled tag. The total length of EIF2AK2 Protein, Human (P. pastoris, His) is 550 a.a., with molecular weight of 64 kDa.

EIF2AK2 蛋白是一种干扰素诱导的 dsRNA 依赖性丝氨酸/苏氨酸蛋白激酶，处于针对病毒感染的先天免疫反应的最前沿。受到病毒攻击后，EIF2AK2 磷酸化真核翻译起始因子 2 (EIF2S1/eIF-2-alpha) 的 α 亚基，启动综合应激反应 (ISR)。这种反应通过将 EIF2S1/eIF-2-α 转化为全局蛋白质合成抑制剂，从而导致细胞和病毒蛋白质合成的关闭，从而起到有效的抗病毒策略的作用。同时，ISR 特异性 mRNA，例如转录激活剂 ATF4，会优先翻译，从而协调多方面的防御机制。EIF2AK2 的抗病毒活性延伸至多种 DNA 和 RNA 病毒，包括 HCV、HBV、MV 和 HHV-1。除了在抗病毒防御中的作用外，EIF2AK2 还调节信号转导、细胞凋亡、细胞增殖和分化。其多功能激酶活性针对 p53/TP53、PPP2R5A、DHX9、ILF3、IRS1 和 HHV-1 病毒蛋白 US11 等底物。值得注意的是，EIF2AK2 的影响延伸到了参与促炎细胞因子表达的各种信号通路（p38 MAP 激酶、NF-kappa-B 和胰岛素信号传导）和转录因子（JUN、STAT1、STAT3、IRF1、ATF3）的调节和干扰素。它作为一种多功能调节剂，对胰岛素信号通路产生积极和消极的影响，并在炎症小体的组装和激活中发挥作用。此外，EIF2AK2 通过与凝溶胶蛋白 (GSN) 结合、调节其活性并影响肌动蛋白动力学（通过相似性）来促进细胞骨架的调节。

Human

P. pastoris

N-6*His

P19525 (A2-C551)

5610  [NCBI]

AGDLSAGFFMEELNTYRQKQGVVLKYQELPNSGPPHDRRFTFQVIIDGREFPEGEGRSKKEAKNAAAKLAVEILNKEKKAVSPLLLTTTNSSEGLSMGNYIGLINRIAQKKRLTVNYEQCASGVHGPEGFHYKCKMGQKEYSIGTGSTKQEAKQLAAKLAYLQILSEETSVKSDYLSSGSFATTCESQSNSLVTSTLASESSSEGDFSADTSEINSNSDSLNSSSLLMNGLRNNQRKAKRSLAPRFDLPDMKETKYTVDKRFGMDFKEIELIGSGGFGQVFKAKHRIDGKTYVIKRVKYNNEKAEREVKALAKLDHVNIVHYNGCWDGFDYDPETSDDSLESSDYDPENSKNSSRSKTKCLFIQMEFCDKGTLEQWIEKRRGEKLDKVLALELFEQITKGVDYIHSKKLIHRDLKPSNIFLVDTKQVKIGDFGLVTSLKNDGKRTRSKGTLRYMSPEQISSQDYGKEVDLYALGLILAELLHVCDTAFETSKFFTDLRDGIISDIFDKKEKTLLQKLLSKKPEDRPNTSEILRTLTVWKKSPEKNERHTC

64 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.