EIF4A2 蛋白, Human (GST)

EIF4A2, an ATP-dependent RNA helicase, is a vital subunit of the eIF4F complex, pivotal in cap recognition and mRNA binding to ribosomes. In translation initiation, EIF4A2 crucially unwinds RNA secondary structures in mRNA 5'-UTRs. This activity facilitates small ribosomal subunit binding and efficient scanning for the initiator codon, orchestrating translation initiation. EIF4A2 Protein, Human (GST) is the recombinant human-derived EIF4A2 protein, expressed by E. coli, with N-GST labeled tag. The total length of EIF4A2 Protein, Human (GST) is 407 a.a., with molecular weight of 73.4 kDa.

EIF4A2 是一种 ATP 依赖性 RNA 解旋酶，作为 eIF4F 复合物中的关键亚基发挥作用，在帽子识别和促进 mRNA 与核糖体的结合中发挥关键作用。在已建立的翻译起始模型中，EIF4A2 承担解旋 mRNA 5'-UTR 中存在的 RNA 二级结构的责任。这种解旋活动对于创造一个有利于小核糖体亚基有效结合和随后扫描起始密码子的环境至关重要，从而协调翻译起始的复杂过程。

Human

E. coli

N-GST

Q14240 (M1-I407)

1974  [NCBI]

MSGGSADYNREHGGPEGMDPDGVIESNWNEIVDNFDDMNLKESLLRGIYAYGFEKPSAIQQRAIIPCIKGYDVIAQAQSGTGKTATFAISILQQLEIEFKETQALVLAPTRELAQQIQKVILALGDYMGATCHACIGGTNVRNEMQKLQAEAPHIVVGTPGRVFDMLNRRYLSPKWIKMFVLDEADEMLSRGFKDQIYEIFQKLNTSIQVVLLSATMPTDVLEVTKKFMRDPIRILVKKEELTLEGIKQFYINVEREEWKLDTLCDLYETLTITQAVIFLNTRRKVDWLTEKMHARDFTVSALHGDMDQKERDVIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTNRENYIHRIGRGGRFGRKGVAINFVTEEDKRILRDIETFYNTTVEEMPMNVADLI

73.4 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.