Granzyme B/GZMB 蛋白, Mouse (HEK293)

Granzyme B/GZMB Protein, Mouse (HEK293) plays essential roles in apoptosis and is involved in inducing inflammation by stimulating cytokine release and is also involved in extracellular matrix remodeling.

颗粒酶 B 在哺乳动物细胞凋亡中发挥重要作用^[1]。颗粒酶 B 是一种丝氨酸蛋白酶，最常见于细胞毒性淋巴细胞 (CTL)、自然杀伤细胞 (NK 细胞) 和细胞毒性 T 细胞的颗粒中。它由这些细胞与成孔蛋白穿孔素一起分泌，以介导靶细胞的凋亡。颗粒酶 B 已被证明可通过刺激细胞因子释放来诱发炎症，并且还参与细胞外基质重塑^[2]。

Measured by its ability to cleave a peptide substrate, t-Butyloxycaronyl-Ala-Ala-Asp-ThioBenzyl ester (Boc-AAD-SBzl), in the presence of 5,5'-Dithio-bis (2-nitrobenzoic acid) (DTNB). The specific activity is 4242 pmol/min/µg as measured under the described conditions.

Mouse

HEK293

Tag Free

P04187 (I21-S247)

14939

IIGGHEVKPHSRPYMALLSIKDQQPEAICGGFLIREDFVLTAAHCEGSIINVTLGAHNIKEQEKTQQVIPMVKCIPHPDYNPKTFSNDIMLLKLKSKAKRTRAVRPLNLPRRNVNVKPGDVCYVAGWGRMAPMGKYSNTLQEVELTVQKDRECESYFKNRYNKTNQICAGDPKTKRASFRGDSGGPLVCKKVAAGIVSYGYKDGSPPRAFTKVSSFLSWIKKTMKSS

Approximately 34.35 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.