1. 重组蛋白
  2. Biotinylated Proteins
  3. HLA-A*0201 WT-1 Complex 蛋白, Human (HEK293, His-Avi)

HLA-A*0201 WT-1 Complex 蛋白, Human (HEK293, His-Avi)

目录号: HY-P77792
COA 产品使用指南

重点介绍了 HLA-A*0201 MAGE-A4 复合蛋白,它是主要组织相容性复合体 (MHC) I 类家族的成员。此外,嵌合 HLA-A*0201 WT-1 复合物四聚体在 MHC I 类家族中得到认可。HLA-A*0201 WT-1 Complex 蛋白, Human (HEK293, His-Avi) 是重组的蛋白二聚体复合物,包含 HLA-A*0201 WT-1 复合蛋白,由 HEK293 表达,带有 C-Avi, C-His 标签。HLA-A*0201 WT-1 Complex 蛋白, Human (HEK293, His-Avi) ,分子量为 52-60 kDa。

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规格 价格 是否有货 数量
20 μg ¥1850 In-stock
50 μg ¥3500 In-stock
100 μg ¥5600 In-stock
> 100 μg   询价  

* Please select Quantity before adding items.

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

HLA-A*0201 MAGE-A4 Complex Protein, a member of the major histocompatibility complex (MHC) class I family, is highlighted. Additionally, the Chimeric HLA-A*0201 WT-1 Complex Tetramer is recognized within the MHC class I family. HLA-A*0201 WT-1 Complex Protein, Human (HEK293, His-Avi) is a recombinant protein dimer complex containing human-derived HLA-A*0201 WT-1 Complex protein, expressed by HEK293 , with C-Avi, C-His labeled tag. HLA-A*0201 WT-1 Complex Protein, Human (HEK293, His-Avi), has molecular weight of 52-60 kDa.

研究背景

嵌合 HLA-A*0201 WT-1 复合物属于主要组织相容性复合物 (MHC) I 类家族。嵌合 HLA-A*0201 WT-1 复合物四聚体也是 MHC I 类家族的成员。

生物活性

Immobilized Human WT-1 (HLA-A*02:01) , His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-WT-1 Antibody, hFc Tag with the EC50 of 10-26.3ng/ml determined by ELISA.

种属

Human

表达系统

HEK293

标签

C-Avi;C-His

蛋白编号

A0A140T913 (G25-T305)&P61769 (I21-M119)&RMFPNAPYL

基因 ID

/&567  [NCBI]

同用名
MHC; WT1; RMF; AWT1; GUD; NPHS4GUD; WAGR; WIT-2; WT33
分子量

52-60 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE.

性状

无菌粉末

组分

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

HLA-A*0201 WT-1 Complex Protein, Human (HEK293, His-Avi) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
HLA-A*0201 WT-1 Complex Protein, Human (HEK293, His-Avi)
目录号:
HY-P77792
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