IL-2R gamma/CD132 蛋白, Human (Biotinylated, HEK293, His-Avi)

IL-2R gamma (CD132), a type I cytokine receptor expressed on leucocyte subsets, is a receptor for IL-2. IL-2R gamma forms heterodimer with IL-2R beta, and increases the affinity of IL-2R beta for IL-2^[1]. IL-2R gamma plays an important role in the development, activation, proliferation, differentiation and regulation of lymphocytes and other cell types^[2]. IL-2R gamma/CD132 Protein, Human (Biotinylated, HEK293, His-Avi) is a biotinylated recombinant human extracellular region of IL-2R gamma (L23-N254) with a C-Terminal Avi and a His tag, which is produced in HEK293 cells.

IL-2R gamma (CD132) 是 IL-2 的一种受体，是 I 型细胞因子受体家族和 5 型亚家族的成员。IL-2R gamma 在白细胞亚群和人类单核细胞中表达^[1][3]。人 IL-2R γ 由胞外结构域 (L23-A262)、螺旋结构域 (V263-L283) 和胞质结构域 (E284-T369) 组成。
不同物种的IL-2Rβ的氨基酸序列不同。人 IL-2R gamma 与小鼠和大鼠的氨基酸序列同一性<75%。
IL-2R γ 对 IL-2 的亲和力较低，但与 IL-2R β 形成异二聚体时，对 IL-2 具有中等亲和力。当 IL-2 浓度相对较高时，IL-2R β/γ 异二聚体复合物会转导信号^[1]。IL-2Rγ可被IL-2、IL-4、IL-7、IL-9、IL-15受体利用，参与淋巴细胞及其他细胞的发育、活化、增殖、分化和调节。类型^[2]。IL-2R gamma 受到 IL-2 和 IFN gamma^[3] 的严格上调。L-2R gamm 突变会导致人类 X 连锁严重联合免疫缺陷 (XSCID)^[4]。
IL-2R gamma 参与炎症反应，并介导细胞活化^[1]。

IL-2R gamma (human) migrates with a molecular weight corresponding to 55–60 kDa (immunoblotting of lysates prepared from Sf9 cells infected with VL1392-hIL-2Rγ)^[5].
IL-2R gamma binds to IL-2 in recombinant IL-2R gamma plasmid (pGEX-4T-1-IL-2Rγ)-transfected 293T cells^[6].

Human

HEK293

C-Avi;C-His

P31785-1 (L23-N254)

3561  [NCBI]

58-70 kDa

Greater than 95% as determined by Tris-Bis PAGE.

<1 EU/μg, determined by LAL method.

• [1]. S Hodge, et al. Surface and intracellular interleukin-2 receptor expression on various resting and activated populations involved in cell-mediated immunity in human peripheral blood. Scand J Immunol. 2000 Jan;51(1):67-75.  [Content Brief]

  [2]. W P Weidanz, et al. Signalling through the IL-2 receptor γ(c) peptide (CD135) is essential for the expression of immunity to Plasmodium chabaudi adami blood-stage malaria.  [Content Brief]

  [3]. M C Bosco, et al. The gamma subunit of the interleukin-2 receptor is expressed in human monocytes and modulated by interleukin-2, interferon gamma, and transforming growth factor beta 1. Blood. 1994 Jun 15;83(12):3462-10.  [Content Brief]

  [4]. K Sugamura, et al. The interleukin-2 receptor gamma chain: its role in the multiple cytokine receptor complexes and T cell development in XSCID. Annu Rev Immunol. 1996;14:179-208.  [Content Brief]

  [5]. K Stenroos, et al. Expression of the mouse interleukin-2 receptor gamma chain in insect cells using a baculovirus expression vector--comparison with the human common gamma chain. Scand J Immunol. 1997 Feb;45(2):140-7.  [Content Brief]

  [6]. Mengmeng Zhao, et al. Expression of Interleukin-2 receptor subunit gamma (IL-2Rγ) and its binding with IL-2 induced activation of CD4 T lymphocytes in flounder (Paralichthys olivaceus). Fish Shellfish Immunol. 2022 Mar;122:426-439.  [Content Brief]