KLHL21 蛋白, Human (Sf9, His, Strep)

KLHL21 acts as a substrate-specific linker in the BCR E3 ubiquitin ligase complex to ensure efficient chromosome alignment and cytokinesis. The BCR(KLHL21) complex coordinates chromosomal passenger complex (CPC) dynamics, directing its transition from chromosomes to the spindle midzone during anaphase. KLHL21 Protein, Human (Sf9, His, Strep) is the recombinant human-derived KLHL21 protein, expressed by Sf9 insect cells , with N-Strep, N-8*His labeled tag. The total length of KLHL21 Protein, Human (Sf9, His, Strep) is 596 a.a., .

KLHL21 作为 BCR (BTB-CUL3-RBX1) E3 泛素-蛋白质连接酶复合物内的底物特异性接头，在确保有效的染色体排列和胞质分裂中发挥着至关重要的作用。BCR(KLHL21) E3 泛素连接酶复合物有助于协调染色体过客复合物 (CPC) 在后期的定位动态，促进其从染色体到纺锤体中区的转变。此外，该复合物积极介导 AURKB 的泛素化，AURKB 是细胞分裂调节的关键参与者。值得注意的是，尽管 BCR(KLHL21) E3 泛素连接酶复合物对 AURKB 进行泛素化，但这不一定会导致其被蛋白酶体降解，这凸显了 KLHL21 控制的蛋白质修饰过程的微妙性质。BCR(KLHL21) 复合物内错综复杂的相互作用强调了其通过精确控制 CPC 定位和 AURKB 泛素化在细胞分裂调节中的关键作用，从而有助于协调重要的细胞事件。

Human

Sf9 insect cells

N-Strep;N-8*His

Q9UJP4 (E2-H597)

9903

69.7 KDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.22 μm filtered solution of 50 mM HEPES (pH 7.5), 500 mM NaCl, 10% glycerol, 1 mM DTT, .

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.