MAN1A2 蛋白, Human (HEK293, His)

The MAN1A2 protein is crucially involved in the maturation of Asn-linked oligosaccharides, which in turn trims α-1,2-linked mannose residues from Man(9)GlcNAc(2) to generate Man(5)GlcNAc(2). During glycosylation, MAN1A2 plays a key role in the progressive trimming of mannose residues that are essential for generating mature glycan structures. MAN1A2 Protein, Human (HEK293, His) is the recombinant human-derived MAN1A2 protein, expressed by HEK293 , with N-His labeled tag. The total length of MAN1A2 Protein, Human (HEK293, His) is 584 a.a., with molecular weight of ~69 KDa.

MAN1A2 蛋白通过顺序修剪 Man(9)GlcNAc(2) 中的 α-1,2-连接甘露糖残基以生成 Man(5)GlcNAc(2)，从而在 Asn 连接寡糖的成熟中发挥至关重要的作用。作为糖基化过程的一部分，MAN1A2 有助于寡糖结构的逐步修饰，在甘露糖残基的逐步修剪中发挥关键作用。这种酶活性对于成熟聚糖结构的生成至关重要，确保糖蛋白的适当功能成熟。MAN1A2 参与寡糖加工凸显了其在控制糖基化和蛋白质成熟的细胞过程的复杂网络中的重要性。

Human

HEK293

N-His;N-10*His

O60476 (P58-R641)

10905  [NCBI]

PDSSKHKRFDLGLEDVLIPHVDAGKGAKNPGVFLIHGPDEHRHREEEERLRNKIRADHEKALEEAKEKLRKSREEIRAEIQTEKNKVVQEMKIKENKPLPPVPIPNLVGIRGGDPEDNDIREKREKIKEMMKHAWDNYRTYGWGHNELRPIARKGHSPNIFGSSQMGATIVDALDTLYIMGLHDEFLDGQRWIEDNLDFSVNSEVSVFEVNIRFIGGLLAAYYLSGEEIFKIKAVQLAEKLLPAFNTPTGIPWAMVNLKSGVGRNWGWASAGSSILAEFGTLHMEFIHLSYLTGDLTYYKKVMHIRKLLQKMDRPNGLYPNYLNPRTGRWGQYHTSVGGLGDSFYEYLLKAWLMSDKTDHEARKMYDDAIEAIEKHLIKKSRGGLTFIGEWKNGHLEKKMGHLACFAGGMFALGADGSRADKAGHYLELGAEIARTCHESYDRTALKLGPESFKFDGAVEAVAVRQAEKYYILRPEVIETYWYLWRFTHDPRYRQWGWEAALAIEKYCRVNGGFSGVKDVYSSTPTHDDVQQSFFLAETLKYLYLLFSGDDLLPLDHWVFNTEAHPLPVLHLANTTLSGNPAVR

Approximately 69 kDa.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.