MEGF10 蛋白, Human (HEK293, His)

The MEGF10 protein is a membrane receptor that plays a key role in phagocytosis by macrophages and astrocytes, specifically the clearance of apoptotic cells through C1q binding to phosphatidylserine. MEGF10 cooperates with ABCA1 to contribute to the formation of large vacuoles and may facilitate amyloid-beta peptide uptake. MEGF10 Protein, Human (HEK293, His) is the recombinant human-derived MEGF10 protein, expressed by HEK293 , with C-His labeled tag.

MEGF10 蛋白是一种膜受体，在巨噬细胞和星形胶质细胞的吞噬作用中发挥着关键作用，特别是在凋亡细胞的清除中。作为“吃我”信号 C1q 的受体，MEGF10 与凋亡细胞表面的磷脂酰丝氨酸结合，促进其吞噬。在此过程中，该蛋白质与 ABCA1 协同作用，有助于形成大的细胞内液泡，可能参与淀粉样蛋白 β 肽的摄取。除了在凋亡细胞清除中的作用外，MEGF10 对于星形胶质细胞介导的小脑发育中凋亡神经元的清除也至关重要。此外，MEGF10影响肌肉细胞的增殖、粘附和运动，通过Notch信号通路在肌生成的调节以及骨骼肌卫星细胞增殖和分化之间的平衡中发挥至关重要的作用。此外，MEGF10 可能有助于视网膜中特定神经元亚型的马赛克间距，确保在视网膜上均匀分布，以进行全面的视野处理。该蛋白形成同聚体并与 GULP1、ABCA1、AP2M1 和 NOTCH1 相互作用，突出了其在各种细胞过程中的多方面作用。

Measured in a cell proliferation assay using C2C12 cells. The ED₅₀ for this effect is 32.22 ng/mL, corresponding to a specific activity is 3.10×10⁴ units/mg.

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  Measured in a cell proliferation assay using C2C12 cells. The ED₅₀ for this effect is 32.22 ng/mL, corresponding to a specific activity is 3.10×10⁴ units/mg.

Human

HEK293

C-10*His

Q96KG7-1 L26-G857)

84466  [NCBI]

LNLEDPNVCSHWESYSVTVQESYPHPFDQIYYTSCTDILNWFKCTRHRVSYRTAYRHGEKTMYRRKSQCCPGFYESGEMCVPHCADKCVHGRCIAPNTCQCEPGWGGTNCSSACDGDHWGPHCTSRCQCKNGALCNPITGACHCAAGFRGWRCEDRCEQGTYGNDCHQRCQCQNGATCDHVTGECRCPPGYTGAFCEDLCPPGKHGPQCEQRCPCQNGGVCHHVTGECSCPSGWMGTVCGQPCPEGRFGKNCSQECQCHNGGTCDAATGQCHCSPGYTGERCQDECPVGTYGVLCAETCQCVNGGKCYHVSGACLCEAGFAGERCEARLCPEGLYGIKCDKRCPCHLENTHSCHPMSGECACKPGWSGLYCNETCSPGFYGEACQQICSCQNGADCDSVTGKCTCAPGFKGIDCSTPCPLGTYGINCSSRCGCKNDAVCSPVDGSCTCKAGWHGVDCSIRCPSGTWGFGCNLTCQCLNGGACNTLDGTCTCAPGWRGEKCELPCQDGTYGLNCAERCDCSHADGCHPTTGHCRCLPGWSGVHCDSVCAEGRWGPNCSLPCYCKNGASCSPDDGICECAPGFRGTTCQRICSPGFYGHRCSQTCPQCVHSSGPCHHITGLCDCLPGFTGALCNEVCPSGRFGKNCAGICTCTNNGTCNPIDRSCQCYPGWIGSDCSQPCPPAHWGPNCIHTCNCHNGAFCSAYDGECKCTPGWTGLYCTQRCPLGFYGKDCALICQCQNGADCDHISGQCTCRTGFMGRHCEQKCPSGTYGYGCRQICDCLNNSTCDHITGTCYCSPGWKGARCDQAGVIIVGNLNSLSRTSTALPADSYQIG

Approximately 95-120 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.