1. 重组蛋白
  2. CD Antigens
  3. Epithelial cell CD Proteins
  4. CD228
  5. Melanotransferrin/CD228 蛋白, Human (HEK293, His)

Melanotransferrin/CD228 蛋白, Human (HEK293, His)

目录号: HY-P700671
COA 产品使用指南

黑素转铁蛋白(CD228)对于细胞铁的吸收至关重要,每个亚基结合一个铁原子。该蛋白质参与细胞膜的内化和再循环,在锌结合方面具有额外的潜在多功能性。Melanotransferrin/CD228 蛋白, Human (HEK293, His) 是重组的 黑素转铁蛋白/CD228 蛋白,由 HEK293 表达,带有 C-His 标签。Melanotransferrin/CD228 蛋白, Human (HEK293, His) 全长 690 个氨基酸,分子量为 78-88 kDa。

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规格 价格 是否有货 数量
20 μg ¥1400 In-stock
50 μg ¥2800 In-stock
100 μg   询价  

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描述

Melanotransferrin, or CD228, is vital for cellular iron uptake, binding a single iron atom per subunit. The protein is involved in internalization and recycling to the cell membrane, with additional potential versatility in zinc binding. Melanotransferrin/CD228 Protein, Human (HEK293, His) is the recombinant human-derived Melanotransferrin/CD228 protein, expressed by HEK293 , with C-His labeled tag. The total length of Melanotransferrin/CD228 Protein, Human (HEK293, His) is 690 a.a., with molecular weight of 78-88 kDa.

研究背景

黑转铁蛋白,也称为 CD228,通过每个亚基结合一个铁原子,在细胞铁吸收中发挥着至关重要的作用。该蛋白质参与内化和随后再循环回细胞膜。值得注意的是,黑转铁蛋白表现出结合锌的能力,表明金属离子相互作用的潜在多功能性。

种属

Human

表达系统

HEK293

标签

C-His

蛋白编号

P08582-1 (G20-C709)

基因 ID

/

蛋白结构
N-term
Melanotransferrin (G20-C709)
Accession # P08582-1
His
C-term
同用名
Melanotransferrin; CD228; MFI2; MELTF; MAP97; MFI2
分子量

78-88 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Melanotransferrin/CD228 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Melanotransferrin/CD228 Protein, Human (HEK293, His)
目录号:
HY-P700671
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