MGMT 蛋白, Human (His)

MGMT Protein crucially defends against O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA, using a suicide reaction to repair methylated nucleobases. It stoichiometrically transfers the methyl group to a cysteine residue, but this process irreversibly inactivates MGMT. The sacrificial nature of MGMT's function emphasizes its commitment to safeguarding cellular DNA integrity at the expense of its own activity. MGMT Protein, Human (His) is the recombinant human-derived MGMT protein, expressed by E. coli , with N-6*His labeled tag. The total length of MGMT Protein, Human (His) is 207 a.a., with molecular weight of ~27.0 kDa.

MGMT 蛋白在细胞防御 DNA 中 O6-甲基鸟嘌呤 (O6-MeG) 和 O4-甲基胸腺嘧啶 (O4-MeT) 的生物效应方面发挥着至关重要的作用。MGMT 通过自杀反应进行操作，通过化学计量将甲基转移到酶内的半胱氨酸残基上，修复 DNA 中的甲基化核碱基。然而，此修复过程会导致 MGMT 蛋白不可逆失活。这种独特的机制强调了 MGMT 功能的牺牲性，强调其以牺牲自身活性为代价来保护细胞 DNA 完整性的承诺。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-6*His

P16455 (M1-N207)

4255  [NCBI]

MDKDCEMKRTTLDSPLGKLELSGCEQGLHEIKLLGKGTSAADAVEVPAPAAVLGGPEPLMQCTAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLKVVKFGEVISYQQLAALAGNPKAARAVGGAMRGNPVPILIPCHRVVCSSGAVGNYSGGLAVKEWLLAHEGHRLGKPGLGGSSGLAGAWLKGAGATSGSPPAGRN

Approximately 27.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl,1 mM DTT,1 mM EDTA,500 mM NaCl,0.1% Trition X-100, pH 8.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.