1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Noggin 蛋白, Mouse (HEK293)

Noggin 蛋白, Mouse (HEK293)

目录号: HY-P700286
COA 产品使用指南

Noggin 是软骨形态发生和关节形成中不可或缺的因子,是骨形态发生蛋白 (BMP) 信号传导的有效抑制剂,在神经管和体节的生长和模式形成中发挥着至关重要的作用。通过形成同型二聚体,Noggin 通过与生长和分化因子(特别是 GDF5 和可能的 GDF6)相互作用,充当抑制软骨细胞分化的关键调节因子。Noggin 蛋白, Mouse (HEK293) 是重组的 头蛋白,由 HEK293 表达,不带标签。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

SPR 检测服务   重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
Free Sample   Apply now  
5 μg ¥435 In-stock
10 μg ¥700 In-stock
50 μg ¥2000 In-stock
100 μg ¥3200 询价
> 100 μg   询价  

* Please select Quantity before adding items.

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

Noggin is an indispensable factor in cartilage morphogenesis and joint formation, a potent inhibitor of bone morphogenetic protein (BMP) signaling, and plays a crucial role in the growth and patterning of the neural tube and somites.By forming homodimers, Noggin acts as a key regulator to inhibit chondrocyte differentiation by interacting with growth and differentiation factors, particularly GDF5 and possibly GDF6.Noggin Protein, Mouse (HEK293) is the recombinant mouse-derived Noggin protein, expressed by HEK293 , with tag free.

研究背景

Noggin 是一种参与软骨形态发生和关节形成的重要蛋白质,在抑制骨形态发生蛋白 (BMP) 信号传导方面发挥着至关重要的作用,而骨形态发生蛋白 (BMP) 对于神经管和体节的生长和模式形成至关重要。作为 BMP 的抑制剂,Noggin 通过与生长和分化因子 5 (GDF5) 以及可能的 GDF6 相互作用,参与软骨细胞分化的调节。Noggin 作为同二聚体存在,直接与 GDF5 相互作用,导致软骨细胞分化受到抑制。这些多方面的功能强调了 Noggin 在胚胎发育中的重要性,特别是在骨骼和神经组织形成的复杂过程中,并强调了它作为 BMP 信号通路关键调节剂的作用(参考:提供的信息)。

生物活性

Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells.The EC50 is 4.6 ng/mL, corresponding to a specific activity of 217391.3043 units/mg.

  • Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 is <4.6 ng/mL, corresponding to a specific activity of >2.2 × 105 units/mg.
种属

Mouse

表达系统

HEK293

标签

Tag Free

蛋白编号

P97466 (Q28-C232)

基因 ID
蛋白结构
N-term
Noggin (Q28-C232)
Accession # P97466
C-term
同用名
NOG; Noggin; SYM1; SYNS1; SYNS1A
氨基酸序列

QHYLHIRPAPSDNLPLVDLIEHPDPIFDPKEKDLNETLLRSLLGGHYDPGFMATSPPEDRPGGGGGPAGGAEDLAELDQLLRQRPSGAMPSEIKGLEFSEGLAQGKKQRLSKKLRRKLQMWLWSQTFCPVLYAWNDLGSRFWPRYVKVGSCFSKRSCSVPEGMVCKPSKSVHLTVLRWRCQRRGGQRCGWIPIQYPIISECKCSC

分子量

Approximately 27-31 kDa due to the glycosylation.

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Noggin Protein, Mouse (HEK293) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
Noggin Protein, Mouse (HEK293)
目录号:
HY-P700286
需求量: