Norrin 蛋白, Human

Norrin protein activates canonical Wnt signaling by binding to FZD4 and LRP5, promotes retinal vascularization and stabilizes β-catenin (CTNNB1). It cooperates with TSPAN12 to independently activate FZD4, suggesting a Wnt-independent mechanism. Norrin Protein, Human is the recombinant human-derived Norrin protein, expressed by E. coli , with tag free. The total length of Norrin Protein, Human is 109 a.a., with molecular weight of ~13 kDa.

Norrin 蛋白作为经典 Wnt 信号通路中的重要参与者，通过与 FZD4 和 LRP5 辅助受体复合物结合来激活该通路。它的重要性在于其在视网膜血管化中的核心作用，因为它作为 FZD4 的配体，导致 β-连环蛋白 (CTNNB1) 的稳定和 LEF/TCF 介导的转录程序的激活。此外，Norrin 蛋白与 TSPAN12 合作独立激活 FZD4，表明存在不依赖于 Wnt 的信号传导机制，该机制也有助于 β-连环蛋白 (CTNNB1) 的积累。此外，诺里蛋白可能参与调节神经细胞分化和增殖的途径，并且它可能在神经外胚层细胞间相互作用中发挥作用。它通过二硫键形成同二聚体，并且是由 TSPAN12、FZD4、LRP5/6 和去甲蛋白本身 (NDP) 组成的复合物的一部分。值得注意的是，Norrin 蛋白表现出与 FZD4 结合的高亲和力，并与 LRP6 相互作用，特别是与其 Beta 螺旋桨 1 和 2 相互作用。

Measured by its binding ability in a functional ELISA. When Human Norrin is coated at 0.5 μg/mL (100 μL/well) can bind Human Frizzled­4. The ED₅₀ for this effect is 86.34 ng/mL.

Human

E. coli

Tag Free

Q00604 (K25-S133)

4693  [NCBI]

KTDSSFIMDSDPRRCMRHHYVDSISHPLYKCSSKMVLLARCEGHCSQASRSEPLVSFSTVLKQPFRSSCHCCRPQTSKLKALRLRCSGGMRLTATYRYILSCHCEECNS

Approximately 13 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 4 mM HCL, pH 2.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.