NucA 蛋白, S. marcescens

The NucA protein plays a central role in cellular processes because it catalyzes the hydrolysis of DNA and RNA (both double- and single-stranded) at the 3' position of the phosphodiester bond to generate 5'-phosphorylated single- and double-stranded , tri- and tetra-nucleotides. This multifunctional enzymatic activity emphasizes the importance of NucA in nucleic acid metabolism, as observed in various studies. NucA Protein, S. marcescens is the recombinant NucA protein, expressed by E. coli , with tag free. The total length of NucA Protein, S. marcescens is 266 a.a., with molecular weight of ~29 kDa.

NucA 是一种催化 DNA 和 RNA 水解的酶，靶向磷酸二酯键的 3' 位并产生 5'-磷酸化的单、二、三和四核苷酸。它对双链和单链核酸均表现出活性，与 RNA 相比，DNA 是稍微更有利的底物。NucA 能够裂解核酸中的磷酸二酯键，从而生成更小的核苷酸片段。这种酶活性表明在核酸代谢中发挥作用，可能有助于细胞内核苷酸成分的周转和再循环。它必须简洁地描述 NucA 的底物特异性及其在磷酸二酯键 3' 位水解 DNA 和 RNA 的能力，强调其在核酸切割中的作用。

Measured by its ability to hydrolyze DNA from salmon testes that incubate at room temperature for 30 minutes. The specific activity is 210792.34 pmol/min/µg.

Others

E. coli

Tag Free

P13717 (M1-N266)

87005285

MRFNNKMLALAALLFAAQASADTLESIDNCAVGCPTGGSSNVSIVRHAYTLNNNSTTKFANWVAYHITKDTPASGKTRNWKTDPALNPADTLAPADYTGANAALKVDRGHQAPLASLAGVSDWESLNYLSNITPQKSDLNQGAWARLEDQERKLIDRADISSVYTVTGPLYERDMGKLPGTQKAHTIPSAYWKVIFINNSPAVNHYAAFLFDQNTPKGADFCQFRVTVDEIEKRTGLIIWAGLPDDVQASLKSKPGVLPELMGCKN

Approximately 29 kDa

Greater than 95% as determined by reducing SDS-PAGE

溶液.

Supplied as a 0.2 μm filtered solution of 50 mM Tris-HCL, 200 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.