PTK9 蛋白, Human

PTK9, an actin-binding protein, regulates motility and morphological processes by inhibiting actin polymerization and capping the barbed ends of filaments. It plays a crucial role in clathrin-mediated endocytosis and the distribution of endocytic organelles. PTK9 interacts with G-actin, capping protein (CP), and possibly TWF2 and phosphoinositides, particularly PI(4,5)P2, which down-regulates its activity. Additionally, it interacts with ACTG1. PTK9 Protein, Human is the recombinant human-derived PTK9 protein, expressed by E. coli , with tag free. The total length of PTK9 Protein, Human is 252 a.a., with molecular weight of ~33 kDa.

PTK9 是一种肌动蛋白结合蛋白，积极参与运动和形态过程，通过可能隔离 G-肌动蛋白对肌动蛋白聚合发挥影响。 PTK9 通过其覆盖细丝带刺末端的能力，在细胞运动中发挥调节作用。此外，它参与网格蛋白介导的内吞作用和内吞细胞器的分布，表明它在细胞动力学中具有多方面的作用。 PTK9 与 G-肌动蛋白（尤其是 ADP-肌动蛋白形式）以及加帽蛋白 (CP) 相互作用，突出了其在肌动蛋白相关过程中的复杂参与。此外，它可能与 TWF2 和磷酸肌醇相互作用，特别是 PI(4,5)P2，与后者结合会导致下调。此外，已观察到 PTK9 与 ACTG1 相互作用，进一步扩展其分子关联网络。

Human

E. coli

Tag Free

Q12792-4 (M1-D252)

5756  [NCBI]

MLYAATRATLKKEFGGGHIKDEVFGTVKEDVSLHGYKKYLLSQSSPAPLTAAEEELRQIKINEVQTDVGVDTKHQTLQGVAFPISREAFQALEKLNNRQLNYVQLEIDIKNEIIILANTTNTELKDLPKRIPKDSARYHFFLYKHSHEGDYLESIVFIYSMPGYTCSIRERMLYSSCKSRLLEIVERQLQMDVIRKIEIDNGDELTADFLYEEVHPKQHAHKQSFAKPKGPAGKRGIRRLIRGPAETEATTD

Approximately 33 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.