RAC2 蛋白, Human (His)

The RAC2 protein is a plasma membrane-associated small GTPase that dynamically regulates cellular responses by cycling between an active GTP-bound state and an inactive GDP-bound state. In its active state, RAC2 regulates secretory function, phagocytosis, and epithelial cell polarization. RAC2 Protein, Human (His) is the recombinant human-derived RAC2 protein, expressed by E. coli , with N-His labeled tag. The total length of RAC2 Protein, Human (His) is 189 a.a., with molecular weight of ~25 kDa.

RAC2 蛋白是一种质膜相关的小 GTP 酶，在其活性 GTP 结合状态和非活性 GDP 结合状态之间动态循环，对各种细胞反应发挥关键的调节控制。在其活性状态下，RAC2 与多种效应蛋白结合，调节分泌功能、凋亡细胞的吞噬作用和上皮细胞极化等过程。值得注意的是，RAC2 在增加 NADPH 氧化酶产生活性氧 (ROS) 方面发挥着至关重要的作用。其活性受到鸟嘌呤核苷酸交换因子 (GEF) 的复杂调控，鸟嘌呤核苷酸交换因子 (GEF) 促进结合的 GDP 交换为游离 GTP、GTP 酶激活蛋白 (GAP)（增强 GTP 水解活性）和 GDP 解离抑制剂（抑制核苷酸从 GTP 解离） GTP酶。这些调节机制突出了 RAC2 在协调细胞过程中的动态性质，并强调了它在细胞内信号级联的复杂平衡中的重要性。

The specific activity was determined to be 8.58 nmol/min/mg in a GTPase-Glo assay using GTP solution substrate.

Human

E. coli

N-His

P15153 (M1-C189)

5880  [NCBI]

MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDSKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASYENVRAKWFPEVRHHCPSTPIILVGTKLDLRDDKDTIEKLKEKKLAPITYPQGLALAKEIDSVKYLECSALTQRGLKTVFDEAIRAVLCPQPTRQQKRAC

Approximately 25 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.