Serum Amyloid A-1 蛋白, Mouse

Serum Amyloid A-1 Protein, a vital acute phase protein, primarily exists as a homohexamer composed of dimers of trimers.Although it can form amyloid fibrils after partial proteolysis, the native, undenatured form does not exhibit amyloid fibril formation in vitro.Serving as an apolipoprotein within the HDL complex, Serum Amyloid A-1 also shows affinity for heparin binding, highlighting its multifaceted role in biological processes.Serum Amyloid A-1 Protein, Mouse is the recombinant mouse-derived Serum Amyloid A-1 protein, expressed by E.coli , with tag free.

血清淀粉样蛋白 A-1 蛋白作为一种重要的急性期蛋白出现，主要以由二聚体或三聚体组成的同六聚体形式存在。虽然它有可能在部分蛋白水解后形成淀粉样蛋白原纤维，但值得注意的是，该蛋白质的天然、未变性形式在体外不会表现出淀粉样蛋白原纤维的形成。此外，血清淀粉样蛋白 A-1 作为 HDL 复合物中的载脂蛋白发挥着至关重要的作用，并表现出与肝素结合的亲和力，强调了其在生物过程中的多方面性质。

Measured by its ability to induce TNF-alpha secretion by J774A.1 mouse reticulum cell sarcoma macrophage cells. The ED₅₀ for this effect is 1.085-7.5 μg/mL.

Mouse

E. coli

Tag Free

P05366 (G20-Y122)

20208  [NCBI]

GFFSFVHEAFQGAGDMWRAYTDMKEANWKNSDKYFHARGNYDAAQRGPGGVWAAEKISDGREAFQEFFGRGHEDTIADQEANRHGRSGKDPNYYRPPGLPDKY

Approximately 12 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4 or PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.