SP-D 蛋白, Human (HEK293)

Surfactant protein-D Protein, Human (HEK293) produced in HEK293 cells is a multimeric collectin that is involved in innate immune defense and expressed in pulmonary, as well as non-pulmonary, epithelia. Surfactant protein-D Protein exerts antimicrobial effects and dampens inflammation through direct microbial interactions and modulation of host cell responses via a series of cellular receptors.

表面活性蛋白-D (SP-D) 参与对吸入微生物和有机抗原的先天反应，并有助于肺内的免疫和炎症调节。SP-D 由肺泡和细支气管上皮细胞合成和分泌，但也由各种外分泌管以及胃肠道和泌尿生殖道粘膜内衬的上皮细胞表达。SP-D 是一种胶原钙依赖性凝集素（或集合素），可与多种微生物表达的表面糖缀合物以及与各种复杂有机抗原表面相关的寡糖结合。SP-D 还与巨噬细胞、中性粒细胞和淋巴细胞表面表达的糖复合物和其他分子发生特异性相互作用。此外，SP-D 与特定的表面活性剂相关脂质结合，可在体外影响含有磷脂酰肌醇的脂质混合物的组织^[1][2]。

Human

HEK293

Tag Free

P35247 (A21-F375)

6441  [NCBI]

AEMKTYSHRTMPSACTLVMCSSVESGLPGRDGRDGREGPRGEKGDPGLPGAAGQAGMPGQAGPVGPKGDNGSVGEPGPKGDTGPSGPPGPPGVPGPAGREGPLGKQGNIGPQGKPGPKGEAGPKGEVGAPGMQGSAGARGLAGPKGERGVPGERGVPGNTGAAGSAGAMGPQGSPGARGPPGLKGDKGIPGDKGAKGESGLPDVASLRQQVEALQGQVQHLQAAFSQYKKVELFPNGQSVGEKIFKTAGFVKPFTEAQLLCTQAGGQLASPRSAAENAALQQLVVAKNEAAFLSMTDSKTEGKFTYPTGESLVYSNWAPGEPNDDGGSEDCVEIFTNGKWNDRACGEKRLVVCEF

40-45 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against PBS.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Sorensen GL. Surfactant Protein D in Respiratory and Non-Respiratory Diseases. Front Med (Lausanne). 2018 Feb 8;5:18.  [Content Brief]

  [2]. Crouch EC. Surfactant protein-D and pulmonary host defense. Respir Res. 2000;1(2):93-108. Epub 2000 Aug 25.  [Content Brief]