Thrombopoietin 蛋白, Cynomolgus (HEK293, His)

TPO/Thrombopoietin Protein is a major regulator of megakaryocyte production and platelet generation, signaling through its receptor Mpl to stimulate the proliferation of megakaryocyte progenitor cells and induce megakaryocyte maturation. Thrombopoietin Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived Thrombopoietin protein, expressed by HEK293 , with C-His labeled tag.

TPO/Thrombopoietin 蛋白是巨核细胞产生的主要调节因子，通过其受体 Mpl 发出信号，刺激巨核细胞祖细胞的增殖并诱导巨核细胞成熟。此外，TPO/Thrombopoietin Protein 可以诱导受体二聚化和酪氨酸磷酸化，以及激活信号传导，如 JAK/STAT、Shc/Ras/MAPK 和 PI3K/Akt^[1]。
在成人造血过程，TPO/Thrombopoietin Protein 维持造血干细胞 (HSC) 的静止状态。并且 TPO/Thrombopoietin Protein信号的丢失与骨髓衰竭和血小板减少有关^[2]。

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED₅₀ for this effect is 14.13 ng/mL, corresponding to a specific activity is 7.077×10⁴ units/mg.

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  Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED₅₀ for this effect is 14.13 ng/mL, corresponding to a specific activity is 7.077×10⁴ units/mg.

Cynomolgus

HEK293

C-6*His

XP_005546625.1(S22-G353)

102144240  [NCBI]

SPAPPACDPRVLSKLLRDSRVLHSRLSLCPEVNPLPTPVLLPAVDFSLGEWKTQMEETKAQDILGAVTLLLEGVMAARGQLGPTCLSSLLGQLSGQVRLLLGALQSLLGTQLPPQGRTTAHKDPNAIFLSFQHLLRGKVRFLLLVGGPTLCVRRAPPTTAVPSRTSLVLTLNELPNRTSGLLETNFTASARTTGSGLQKRQQGFRAKIPGLLNQTSRSLDQIPGYLNRIHKLLNGTRGLFPGPSRRTLGAPDISPGTSDTGSLPPNLQPGYSPSPTHPPTGQYTLFPLPPTLPSPVVQLHPLLPDPSAPTPTPTSPLLNTSHTHSQNLSQEG

Approximately 70-96 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.