TMED9 蛋白, Human (HEK293, His)

TMED9, a key player in vesicular protein trafficking, operates prominently in the early secretory pathway, specifically in COPI vesicle-mediated retrograde transport. It facilitates coatomer recruitment to membranes, enhances ARFGAP2 activity, and ensures specific retention of p24 complexes in cis-Golgi membranes, notably influencing TMED2 and TMED10 localization. Beyond retrograde transport, TMED9 contributes to intracellular membrane organization, including the ER-Golgi intermediate compartment and Golgi apparatus, and participates in PTPN2 isoform PTPB ER localization. TMED9's dynamic oligomeric states involve monomers and homodimers, influenced by its interactions with EMP24/GP25L family members (TMED2, TMED7, and TMED10) and specific associations with TMED5, COPG1, PTPN2, SPAST, and STX17. TMED9 Protein, Human (HEK293, His) is the recombinant human-derived TMED9 protein, expressed by HEK293, with C-His labeled tag. The total length of TMED9 Protein, Human (HEK293, His) is 165 a.a., with molecular weight of ~20.8 kDa.

TMED9 是一种与囊泡蛋白运输密切相关的蛋白质，主要在早期分泌途径中发挥作用，特别是在 COPI 囊泡介导的逆行运输中，促进外衣异构体招募到膜上。它增强了 ARFGAP2 的外壳依赖性活性，并在 p24 复合物在顺式高尔基体膜中的特异性保留中发挥关键作用，特别有助于 TMED2 和 TMED10 在顺式高尔基体网络中的耦合定位。除了参与逆行运输之外，TMED9 还参与细胞内膜的组织，包括 ER-高尔基体中间室和高尔基体。它进一步参与 PTPN2 同种型 PTPB 的 ER 定位。TMED9 表现出动态寡聚状态，在内质网中以单体和同二聚体形式存在，在内质网-高尔基体中间室和顺式高尔基体网络中主要呈单体状态。EMP24/GP25L 家族的其他成员（例如 TMED2、TMED7 和 TMED10）可能发生寡聚化。此外，TMED9 与 TMED5、COPG1、PTPN2、SPAST 和 STX17 发生特定的相互作用，强调了它在细胞内过程中的多方面参与。

Measured in a cell proliferation assay using MCF-7 cells. The ED₅₀ for this effect is 19.86 ng/mL. Corresponding to a specific activity is 5.035×10⁴ Unit/mg.

Human

HEK293

C-His

Q9BVK6/NP_059980.2 (L38-R202)

54732  [NCBI]

LYFHIGETEKKCFIEEIPDETMVIGNYRTQLYDKQREEYQPATPGLGMFVEVKDPEDKVILARQYGSEGRFTFTSHTPGEHQICLHSNSTKFSLFAGGMLRVHLDIQVGEHANDYAEIAAKDKLSELQLRVRQLVEQVEQIQKEQNYQRWREERFRQTSESTNQR

Approximately 20.8 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.