TNF-alpha/TNFSF2 蛋白, Cynomolgus (N-His)

TNF-alpha/TNFSF2 protein binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It induces tumor cell death, fever, cachexia, cell proliferation, and cell differentiation. It also causes insulin resistance and GKAP42 protein degradation in adipocytes. Additionally, it plays a role in angiogenesis, osteoclastogenesis, and IL12 production in dendritic cells. TNF-alpha/TNFSF2 Protein, Cynomolgus (N-His) is the recombinant cynomolgus-derived TNF-alpha/TNFSF2 protein, expressed by E. coli , with N-6*His labeled tag.

TNF-α/TNFSF2 蛋白是一种细胞因子，与 TNFRSF1A/TNFR1 和 TNFRSF1B/TNFBR 结合，主要由巨噬细胞分泌，具有多种生物学功能。它具有诱导特定肿瘤细胞系细胞死亡的能力，作为一种有效的热原，可以通过直接作用或刺激白细胞介素-1 分泌而引起发烧，并参与恶病质的诱导。在一定条件下，TNF-α/TNFSF2既能起到刺激细胞增殖的作用，又能起到诱导细胞分化的作用。它还通过抑制胰岛素诱导的 IRS1 酪氨酸磷酸化和葡萄糖摄取来促进脂肪细胞中的胰岛素抵抗，并对 GKAP42 蛋白降解产生额外影响。此外，TNF-α/TNFSF2通过与IL1B和IL6协同诱导VEGF产生来参与血管生成，并促进破骨细胞生成，从而介导骨吸收。TNF-α 的胞内结构域 (ICD) 形式可诱导树突状细胞产生 IL12，进一步凸显其对不同细胞过程的多方面影响。

Measured in a cytotoxicity assay using L929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED₅₀ this effect is 11.55 ng/mL, corresponding to a specific activity is 8.658×10⁴ units/mg.

• 
  Measured in a cytotoxicity assay using L929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED₅₀ this effect is 11.55 ng/mL, corresponding to a specific activity is 8.658×10⁴ units/mg.

Cynomolgus

E. coli

N-6*His

P79337/NP_001272206.1 (V77-L233)

102139631

VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALVANGVELTDNQLVVPSEGLYLIYSQVLFKGQGCPSNHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINLPDYLDFAESGQVYFGIIAL

Approximately 19 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, 500 mM arginine, pH 8.0, 10% Glycerol.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.