Wee1 蛋白, Human (Sf9, His)

Wee1 Protein acts as a crucial negative regulator of the G2 to M transition, ensuring nuclear protection by phosphorylating CDK1 on 'Tyr-15' and inactivating the cyclin B1-CDK1 complex. Its peak activity in G2 phase decreases as cells progress into M phase, with dynamic regulation, increased phosphorylation during S and G2 phases, and diminished levels during M/G1 phase transition potentially due to degradation. Wee1 Protein, Human (Sf9, His) is the recombinant human-derived Wee1 protein, expressed by Sf9 insect cells , with N-8*His labeled tag. The total length of Wee1 Protein, Human (Sf9, His) is 285 a.a., .

Wee1 蛋白作为 G2 到 M 转变的关键负调节因子，通过保护细胞核免受细胞质结合的细胞周期蛋白 B1 复合物 CDK1 的激活来防止进入有丝分裂。通过“Tyr-15”上 CDK1 的磷酸化，Wee1 特异性靶向并灭活细胞周期蛋白 B1-CDK1 复合物，其活性在 G2 期达到峰值，并在细胞进入 M 期时达到最低值。值得注意的是，Wee1 对细胞周期蛋白 B1-CDK1 的磷酸化仅发生在“Tyr-15”上，而单体 CDK1 保持未磷酸化。其活性经历动态调节，在S期和G2期增加，在M期减少，并伴有过度磷酸化。此外，Wee1 蛋白水平在 M/G1 相变期间发生相关降低，可能归因于其降解。

Human

Sf9 insect cells

N-8*His

P30291 (M291-K575)

7465

MKSRYTTEFHELEKIGSGEFGSVFKCVKRLDGCIYAIKRSKKPLAGSVDEQNALREVYAHAVLGQHSHVVRYFSAWAEDDHMLIQNEYCNGGSLADAISENYRIMSYFKEAELKDLLLQVGRGLRYIHSMSLVHMDIKPSNIFISRTSIPNAASEEGDEDDWASNKVMFKIGDLGHVTRISSPQVEEGDSRFLANEVLQENYTHLPKADIFALALTVVCAAGAEPLPRNGDQWHEIRQGRLPRIPQVLSQEFTELLKVMIHPDPERRPSAMALVKHSVLLSASRK

34.2 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.22 μm filtered solution of 50 mM HEPES, 200 mM NaCl, 20% glycerol, 1 mM DTT, pH 7.5.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.