ZNRF3 蛋白, Human (HEK293, His)

ZNRF3, as an E3 ubiquitin protein ligase, plays a crucial role as a negative regulator in the Wnt signaling pathway. It does this by mediating the ubiquitination and subsequent degradation of components within the Wnt receptor complex, namely Frizzled and LRP6. ZNRF3 Protein, Human (HEK293, His) is the recombinant human-derived ZNRF3 protein, expressed by HEK293 , with C-6*His labeled tag.

ZNRF3 是一种 E3 泛素蛋白连接酶，充当 Wnt 信号通路的重要负调节因子。通过介导 Wnt 受体复合物中关键成分（包括 Frizzled 和 LRP6）的泛素化和随后的降解，ZNRF3 在调节经典和非经典 Wnt 信号通路中发挥着关键作用。特别是，在肠道干细胞区，ZNRF3通过抑制Wnt信号传导发挥肿瘤抑制因子的作用，从而限制肠道干细胞区的大小。这种调节功能强调了其在控制细胞过程中的重要性。ZNRF3 与 RSPO2 和 RNF43 一起构成了控制肢体规格的主开关，强调了其在发育途径中更广泛的参与。

Measured by its binding ability in a functional ELISA. Immobilized ZNRF3 Protein, Human (HEK293, His) at 5 μg/mL can bind Human RSPO2, the ED₅₀ is ≤10 ng/mL.

Human

HEK293

C-6*His

Q9ULT6-1 (K56-M219)

84133  [NCBI]

KETAFVEVVLFESSPSGDYTTYTTGLTGRFSRAGATLSAEGEIVQMHPLGLCNNNDEEDLYEYGWVGVVKLEQPELDPKPCLTVLGKAKRAVQRGATAVIFDVSENPEAIDQLNQGSEDPLKRPVVYVKGADAIKLMNIVNKQKVARARIQHRPPRQPTEYFDM

Approximately 21 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized a 0.22 μm filtered solution of PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.