1. 糖生物学酶
  2. β1-4 Galactosidase, E. coli

β1-4 Galactosidase, E. coli  (Synonyms: β1-4 半乳糖苷酶)

目录号: HY-P2869B
COA 产品使用指南

β1-4 Galactosidase, E. coli 是一种高度特异性的外切糖苷酶,可催化寡糖中末端非还原性 β1-4 连接的半乳糖残基的水解。

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β1-4 Galactosidase, E. coli Chemical Structure

β1-4 Galactosidase, E. coli Chemical Structure

CAS No. : 9031-11-2

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100 U ¥1200
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Other Forms of β1-4 Galactosidase, E. coli:

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

β1-4 Galactosidase, E. coli is a highly specific exoglycosidase that catalyzes the hydrolysis of terminal, non-reducing β1-4 linked galactose residues from oligosaccharides[1].

IC50 & Target

others

体外研究
(In Vitro)

使用方法 1) 取 1 μg 糖蛋白或者 100 nM 寡糖样品,加纯化水至反应体系为9μL。
2) 加入 1 μL 反应buffer (推荐 50 mM CaCl2, 500 mM sodium acetate, pH 5.5 at 25℃),使反应总体积为 10 μL。
3) 加入 1 μL β1-4 Galactosidase,轻柔混匀。
4) 37℃ 条件下反应 1 小时。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

CAS 号
性状

液体

颜色

Colorless to light yellow

中文名称

β1-4 半乳糖苷酶

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

纯度 & 产品资料
参考文献

β1-4 Galactosidase, E. coli 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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β1-4 Galactosidase, E. coli
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HY-P2869B
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