360A

目录号: HY-15595: FAQs
Data Sheet 产品使用指南

摩尔计算器

360A 是一种 G-四联体 (G-quadruplex) 的稳定剂，同时可抑制端粒酶 (telomerase) 的活性，在 TRAP-G4 实验中，IC₅₀ 值为 300 nM。

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360A Chemical Structure

CAS No. : 794458-56-3

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规格	价格	是否有货
1 mg	￥1100	1 - 2 周
5 mg	￥2550	1 - 2 周
10 mg		询价
50 mg		询价

or 大包装询价

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Other Forms of 360A:

360A iodide In-stock

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生物活性
实验参考方法
纯度 & 产品资料
参考文献

生物活性

360A is a selective stabilizer of G-quadruplex, and also inhibits telomerase activity with an IC₅₀ of 300 nM for telomerase in TRAP-G4 assay.

IC₅₀ & Target

IC50: 300 nM (Telomerase)^[1]
G-quadruplex^[1]

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
HEK-293T	IC₅₀	45 nM Compound: 360A	Inhibition of human telomerase expressed in in HEK293T cells in presence of primer (T2AG3)4 by primer extension assay Inhibition of human telomerase expressed in in HEK293T cells in presence of primer (T2AG3)4 by primer extension assay	[PMID: 17954919]
HEK-293T	IC₅₀	52 nM Compound: 360A	Inhibition of human telomerase expressed in HEK293T cells treated before telomerase elongation by telomeric repeat amplification protocol assay Inhibition of human telomerase expressed in HEK293T cells treated before telomerase elongation by telomeric repeat amplification protocol assay	[PMID: 17954919]
HEK-293T	IC₅₀	5600 nM Compound: 360A	Inhibition of human telomerase expressed in HEK293T cells in presence of primer (T2AG3)3 by primer extension assay Inhibition of human telomerase expressed in HEK293T cells in presence of primer (T2AG3)3 by primer extension assay	[PMID: 17954919]
HEK-293T	IC₅₀	63 nM Compound: 360A	Inhibition of human telomerase expressed in HEK293T cells treated after telomerase elongation by telomeric repeat amplification protocol assay Inhibition of human telomerase expressed in HEK293T cells treated after telomerase elongation by telomeric repeat amplification protocol assay	[PMID: 17954919]
HEK-293T	IC₅₀	8400 nM Compound: 360A	Inhibition of human telomerase expressed in HEK293T cells in presence of primer TS by primer extension assay Inhibition of human telomerase expressed in HEK293T cells in presence of primer TS by primer extension assay	[PMID: 17954919]

体外研究
(In Vitro)

360A inhibits telomerase activity and stabilizes G-quadruplex, with an IC₅₀ of 300 nM for telomerase in TRAP-G4 assay. 360A reduces the viability of glioma cell lines, such as T98G, CB193, U118-MG, SAOS-2 and Primary astrocytes, with IC₅₀s of 4.8 ± 1.1 μM, 3.9 ± 0.4 μM, 8.4 ± 0.5 μM, >15 μM and 17.4 ± 1.2 μM, respectively^[1]. 360A causes Rad51-dependent telomere aberrations preferentially involving the lagging strand telomeres, including telomere losses or telomere doublets, and induces DNA-PKcs-dependent sister telomere fusions^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

449.50

Formula

C₂₇H₂₃N₅O₂

CAS 号

794458-56-3

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

纯度 & 产品资料

产品使用指南 (1538 KB)

参考文献

[1]. Pennarun G, et al. Apoptosis related to telomere instability and cell cycle alterations in human glioma cells treated by new highly selective G-quadruplex ligands. Oncogene. 2005 Apr 21;24(18):2917-28. [Content Brief]

[2]. Gauthier LR, et al. Rad51 and DNA-PKcs are involved in the generation of specific telomere aberrations induced by the quadruplex ligand 360A that impair mitotic cell progression and lead to cell death. Cell Mol Life Sci. 2012 Feb;69(4):629-40. [Content Brief]

Cell Assay ^[1]	The cell proliferation reagent WST-1 assay is performed. In brief, cells are seeded at various densities, depending on cell type (0.25-4 × 10³ cells/well in 100 μL complete medium), in 96-well culture plates and treated with various concentrations (0.1-20 μM) of 360A or the corresponding concentrations of DMSO (control wells) for 3 or 7 days at 37°C in an atmosphere containing 5% CO₂. For 7-day assays, the medium is changed on day 3. Experiments are performed in triplicate^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Pennarun G, et al. Apoptosis related to telomere instability and cell cycle alterations in human glioma cells treated by new highly selective G-quadruplex ligands. Oncogene. 2005 Apr 21;24(18):2917-28. [Content Brief] [2]. Gauthier LR, et al. Rad51 and DNA-PKcs are involved in the generation of specific telomere aberrations induced by the quadruplex ligand 360A that impair mitotic cell progression and lead to cell death. Cell Mol Life Sci. 2012 Feb;69(4):629-40. [Content Brief]

Cell Assay
^[1]

The cell proliferation reagent WST-1 assay is performed. In brief, cells are seeded at various densities, depending on cell type (0.25-4 × 10³ cells/well in 100 μL complete medium), in 96-well culture plates and treated with various concentrations (0.1-20 μM) of 360A or the corresponding concentrations of DMSO (control wells) for 3 or 7 days at 37°C in an atmosphere containing 5% CO₂. For 7-day assays, the medium is changed on day 3. Experiments are performed in triplicate^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

[1]. Pennarun G, et al. Apoptosis related to telomere instability and cell cycle alterations in human glioma cells treated by new highly selective G-quadruplex ligands. Oncogene. 2005 Apr 21;24(18):2917-28. [Content Brief]

[2]. Gauthier LR, et al. Rad51 and DNA-PKcs are involved in the generation of specific telomere aberrations induced by the quadruplex ligand 360A that impair mitotic cell progression and lead to cell death. Cell Mol Life Sci. 2012 Feb;69(4):629-40. [Content Brief]

360A 相关分类

摩尔计算器
稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量		浓度		体积		分子量 *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)	×	体积 _(start)	=	浓度 _(final)	×	体积 _(final)
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

360A794458-56-3G-quadruplexTelomeraseInhibitorinhibitorinhibit

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360A

Other Forms of 360A:

MCE 顾客使用本产品发表的 2 篇科研文献

360A 相关产品

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纯度 & 产品资料

参考文献

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The dilution calculator equation

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360A

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360A 相关抗体

MCE 顾客使用本产品发表的 2 篇科研文献

360A 相关产品

•同靶点产品:

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生物活性

实验参考方法

纯度 & 产品资料

参考文献

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The molarity calculator equation

The dilution calculator equation

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