C-Phycocyanin  (Synonyms: C-藻蓝蛋白; C-PC)

A549 cells are divided into four groups: (1) control group: the cells are not treated with any drug. (2) C-phycocyanin (C-PC) treated group: the cells are treated with C-phycocyanin alone. (3) All-trans retinoic acid (ATRA) treated group: the cells were treated with ATRA alone. (4) C-phycocyanin+ATRA combined group: the cells are treated simultaneously with C-phycocyanin and ATRA. For the combined treatment group, the combination index (CI) between two drugs is calculated. CI values of less than, equal to, and more than 1 indicate synergy, additivity, and antagonism, respectively^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

A total of 40 adult NU/NU mice (20 males and 20 females, 20 to 22 g) are used for the study. The mouse tumor models are set up by subcutaneous injection of 2×10⁶ A549 cells near the armpit area. Fifteen days later, the mice are randomly divided into four groups: control group, All-trans retinoic acid (ATRA) treatment group which is treated with 0.2 mL ATRA (10 mM), C-phycocyanin treatment group which is given 0.2 mL C-phycocyanin (320 mg/mL), and C-phycocyanin+ATRA treatment group receiving 0.2 mL C-phycocyanin (320 mg/mL) and 0.2 mL ATRA (10 mM) at the same time. These agents are injected into the area of tumors and the duration of drug treatment is 10 days. Two days after drug withdrawal, the mice are executed, and then tumors and spleens are picked out^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

• [1]. Li B, et al. The synergistic antitumor effects of all-trans retinoic acid and C-phycocyanin on the lung cancer A549 cells in vitro and in vivo. Eur J Pharmacol. 2015 Feb 15;749:107-14.  [Content Brief]