alpha-1,4-Galactosyltransferase (LgtC)  (Synonyms: α-1,4-半乳糖基转移酶 (LgtC); A4GALT)

alpha-1,4-Galactosyltransferase (LgtC) (A4GALT) is a glycosphingolipid-specific glycosyltransferase. alpha-1,4-Galactosyltransferase (LgtC) transfers a galactose to the alpha-1,4 position of lactosylceramide to form globotriaosylceramide. alpha-1,4-Galactosyltransferase (LgtC) can be used for the synthesis of P1 blood group antigens^[1][2].

产品信息
本品利用大肠杆菌重组表达，N-terminal His-tag，分子量 32 kDa，缓冲液组分 20 mM Tris-HCl , pH 7.5, 5% glycerol，可用于将半乳糖转移到 以 Gal 为非还原端的糖链上。

使用说明
1.按照反应体系加入底物，5 mM MgCl₂,1 mM DTT，可适当加入缓冲盐 (如Tris-HCl)，调节pH为7.5，加入适量LgtC (根据反应需求)；
2.混匀后，将样品置于 37 ℃ 孵育2-4 h。

注意事项
体系放大时孵育时间或酶量需要根据实际情况调整，可低速震荡混匀。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

52725-57-2

液体

Colorless to light yellow

α-1,4-半乳糖基转移酶 (LgtC)

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Kaczmarek R, et al. Evaluation of an amino acid residue critical for the specificity and activity of human Gb3/CD77 synthase. Glycoconj J. 2016 Dec;33(6):963-973.  [Content Brief]

  [2]. Iwamura K, et al. The blood group P1 synthase gene is identical to the Gb3/CD77 synthase gene. A clue to the solution of the P1/P2/p puzzle. J Biol Chem. 2003 Nov 7;278(45):44429-38.  [Content Brief]