APC6 抗体

APC6 Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 72 kDa, targeting to APC6. It can be used for WB,ICC/IF assays with tag free, in the background of Human, Rat.

Free

Predicted band size: 72 kDa

Protein A affinity purified.

Cytoplasm.

Non-conjugated

Unmodified

Cell Biology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Rat

SwissProt: Q13042 Human;SwissProt: Q8R349 Mouse;Unigene: 66098 Rat

WB: 1:1,000-1:2,000 ;ICC/IF: 1:50-1:200

• 
  Western blot analysis of extracts from HepG2(lane 2(10μg) or (lane 3(20μg)) and Hela(lane 4(10μg) or (lane 5(20μg)), using APC6 (HY-P80017) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80017, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (HY-P8001/HY-P8004, 1/10,000) was used for 1 hour at room temperature.

WB, ICC/IF

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human APC6.AA range:181-247.