ATF6 抗体 (YA604)

ATF6 Antibody (YA604) is a non-conjugated and Rabbit origined monoclonal antibody about 75 kDa, targeting to ATF6. It can be used for WB,IHC-P,IP assays with tag free, in the background of Human.

ATF6：该基因编码的转录因子可在内质网 (ER) 应激期间激活未折叠蛋白反应 (UPR) 的靶基因。虽然它是一种转录因子，但这种蛋白质的不同寻常之处在于它是作为嵌入 ER 中的跨膜蛋白合成的。它作为 ER 应激传感器/转换器发挥作用，在 ER 应激诱导的蛋白水解后，它通过存在于编码 ER 伴侣蛋白的基因启动子中的顺式作用 ER 应激反应元件 (ERSE) 作为核转录因子发挥作用。这种蛋白质已被确定为静止而非增殖性鳞状癌细胞的存活因子。关于该基因多态性与不同人群糖尿病相关性的报道相互矛盾，但另一种多态性与血浆胆固醇水平升高有关。该基因也被认为是囊性纤维化的潜在治疗靶点。[由 RefSeq 提供，2011 年 8 月]

Free

22926

Predicted band size: 75 kDa

affinity purified

Non-conjugated

Unmodified

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

P18850

WB: 1:500-1:1,000 ;IHC: 1:50-1:100 ;IP: 1:20

• 
  Western blot analysis of extracts from Hela using ATF6 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

WB, IHC-P, IP

液体

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human ATF6.The exact sequence is proprietary to MCE.