B-Raf 抗体

B-Raf Antibody is a non-conjugated and Mouse origined monoclonal antibody about 84 kDa, targeting to B-Raf. It can be used for WB,IHC-P,ICC assays with tag free, in the background of Human, Mouse, Rat.

Free

Predicted band size: 84 kDa

Protein A affinity purified.

Nucleus, Cytoplasm, Cell membrane.

Non-conjugated

Unmodified

Cell Biology

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Mouse, Rat

SwissProt: P15056 Human;SwissProt: P28028 Mouse

WB: 1:500-1:2,000 ;ICC: 1:100-1:500 ;IHC-P: 1:100-1:500

• 
  Western blot analysis of extracts from K562(lane 2(20μg), HT-29(lane 3(20μg) and HEK293(lane 4(20μg) ) using B-Raf (HY-P80036) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80036, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

WB, IHC-P, ICC/IF

液体

Supplied in 1*PBS (pH7.4), 0.2% BSA and 50% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG2b

Endogenous

Synthetic peptide corresponding to Human B-Raf.AA range:49-239.