BRAF 抗体

BRAF Antibody is a non-conjugated and Mouse origined monoclonal antibody about 84 kDa, targeting to BRAF (4E1). It can be used for WB assays with tag free, in the background of Human, Mouse.

BRAF (4E1)：该基因编码属于 RAF 丝氨酸/苏氨酸蛋白激酶家族的蛋白质。该蛋白在调节 MAP 激酶/ERK 信号通路中发挥作用，该通路影响细胞分裂、分化和分泌。该基因的突变，最常见的是 V600E 突变，是黑色素瘤中最常发现的致癌突变，也已在各种其他癌症中发现，包括非霍奇金淋巴瘤、结直肠癌、甲状腺癌、非小细胞癌肺癌、毛细胞白血病和肺腺癌。该基因的突变也与表现出重叠表型的心面皮肤、Noonan 和 Costello 综合征有关。已在 X 染色体上鉴定出该基因的假基因。[由 RefSeq 提供，2017 年 8 月]

Free

673

Predicted band size: 84 kDa

affinity purified

Non-conjugated

Unmodified

Cell Biology

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Mouse, Rat

P26367

WB: 1:500-1:1000 ;IHC: 1:50-1:100 ;IF: 1:50-1:200

• 
  Western blot analysis of extracts from Hela (lane 1) and NIH3T3 (lane 2) and HCT116 (lane 3) and 293T (lane 4) using BRAF antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

WB

液体

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG2a

Endogenous

Synthetic peptide corresponding to Human B Raf.The exact sequence is proprietary to MCE.